2,172 results
-
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterZic2a enhancer driving c-fos promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-Cre
Plasmid#122562PurposeIntegration of Cre sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-Cre-SV40pA
UseCloningAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2_(-3kb)nrg1_typeII:GFP-CAAX
Plasmid#199394PurposepDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region controlDepositorInserts(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR
UseTol2 gateway destination vectorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; T3_gRNA
Plasmid#199335PurposepDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNADepositorInsertszCREST3
Cas9
nrg1 type III sgRNA
UseTol2 destination vectorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0005
Plasmid#42245PurposegRNA targeted to zebrafish gene gsk3bDepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-U6ac-cdk2-guides
Plasmid#168250Purpose"neutrophil specific GFP with ubiquitous cdk2 sgRNAs"DepositorInsertcdk2 sgRNAs
UseCRISPRAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pPRISM-Stop-cmlc2-TagBFP
Plasmid#117774PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
M16 pCS-twhh-bGFP
Plasmid#17153DepositorInserttwhh promoter (shhb Zebrafish)
TagsbGFPAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0008
Plasmid#42248PurposegRNA targeted to zebrafish gene tia1lDepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS-foxc1a-2a-nlstdTomato
Plasmid#132970Purposefull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vectorDepositorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Bin1a
Plasmid#176013PurposeMammalian expression of zebrafish EGFP-Bin1a. Parton lab clone HYADepositorInsertBin1a
TagsEGFPExpressionMammalianAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
CACNA1F_L (OZ507)
Plasmid#26957DepositorInsertZinc finger array targeting cacna1f (cacna1f Zebrafish)
UseZebrafish targetingAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
pT2-cryR;mafbbCE1-basEgfp
Plasmid#90137Purposecontains mafbb enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertmafbbE1basEgfp
TagsEGFPExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-TagBFP
Plasmid#117783PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
M28 Nac>GFP
Plasmid#17178DepositorInsertnacre promoter (mitfa Zebrafish)
TagsGFPAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pME-foxc1a
Plasmid#132971Purposefull-length of foxc1a without stop codon in pmE Gateway vectorDepositorInsertfoxc1a (foxc1a Zebrafish)
UseGateway vectorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-LA-nanoLuc-RA
Plasmid#128692PurposeDonor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locusDepositorInsertapobb.1 NanoLuc
UseMultisite-gateway assemblyTagsNanoLucAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
459 pME-etv2
Plasmid#49002Purposefull length coding sequence for zebrafish etv2 gene in gateway middle entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway middle entryAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-gcry-TagBFP
Plasmid#117789PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj10a-IRES-EGFP
Plasmid#164947PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFPDepositorInsertkcnj10a (kcnj10a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SI4-Jamc.2
Plasmid#28216DepositorInsertJamc.2 mouse monoclonal antibody (jam3a Zebrafish)
ExpressionMammalianAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-zDrosha
Plasmid#171540PurposeEncodes Zebrafish Drosha proteinDepositorInsertdrosha (rnasen Zebrafish)
ExpressionMammalianAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab32a
Plasmid#109689PurposeMultisite gateway vector for 3' tagging with rab32aDepositorInsertrab32a (rab32a Zebrafish)
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
M17 ptwhh-IGFP65C
Plasmid#17154DepositorInserttwhh promoter (shhb Zebrafish)
Tagsb-globin intron, GFPAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfTpo
Plasmid#64309PurposeExpress soluble His-tagged zebrafish thrombopoietin in insect cellsDepositorInsertThrombopoietin (thpo Zebrafish)
Tags(His)6ExpressionInsectMutationThrombopoietin starts at AA37 (AA1-36 encode the …Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only