2,226 results

1. pOTTC763 - pX458 with rat Rosa26 gRNA A

   Plasmid

   #113161

   Purpose

   A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat Rosa26
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-LAMP1-EGFP

   Plasmid

   #169003

   Purpose

   Express rat LAMP1-EGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

   Insert

   rat LAMP1-EGFP
   

   Use

   Unspecified

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-jGCaMP7b-WPRE

   Plasmid

   #104489

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-Flag-Rat Arc-Arc- Genomic 3'UTR

   Plasmid

   #233053

   Purpose

   To express Flag-tagged Rat Arc from a CMV promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns

   Depositor

   Jonathan Ploski

   Insert

   Rat Arc (Arc Rat)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-AAV-sh[SNCA]

   Plasmid

   #75437

   Purpose

   knockdown alpha-synuclein in rat neurons

   Depositor

   Edward Burton

   Article

   Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

   Insert

   snca (Snca Rat)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Col2.3deltaTK

   Plasmid

   #114273

   Purpose

   Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

   Depositor

   David Rowe

   Article

   Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

   Insert

   Col2.3 promoter + deltaTK + BGH polyA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   2.3 kb rat type 1 collagen

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt

   Plasmid

   #175180

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Has Service

   AAV1

   Insert

   pAAV hSyn FLEX iGluSnFR3 v857.PDGFR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pKH3-p190

   Plasmid

   #15547

   Purpose

   Expression of HA-tagged p190 RhoGAP

   Depositor

   Ian Macara

   Article

   Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

   Insert

   p190 GTPase activating protein (Arhgap35 Rat)
   

   Use

   Tags

   Triple HA1 tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

10. CMV-mEGFP-CREB

    Plasmid

    #137001

    Purpose

    mEGFP-CREB donor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.iGluSnFR3.v857.SGZ

    Plasmid

    #178334

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA GST PreScission p70 S6K1

    Plasmid

    #15511

    Depositor

    David Sabatini

    Article

    Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

    Insert

    p70 S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

13. myc-mTOR (1-1482 aa)

    Plasmid

    #21745

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1-1482) (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1-1482

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

14. N-WASP

    Plasmid

    #186132

    Purpose

    For expression of recombinant N-WASp in bacteria cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    WASL (WASL Rat)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Amino Acid residues 183-193 fused to 273-501

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-SYP1-miniSOG-T2A-mCherry

    Plasmid

    #50972

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-myc-Wnk1

    Plasmid

    #38779

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. AAV-mDlx-jGCaMP8f-WPRE

    Plasmid

    #176753

    Purpose

    jGCaMP8f expression in GABA-ergic interneurons under the control of mDlx enhancer

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    mDlx

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBa-KIF5C 559-tdTomato-FKBP

    Plasmid

    #64211

    Purpose

    KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Kif5c (Kif5c Rat)
    

    Use

    Tags

    FKBP and tdTomato

    Expression

    Mammalian

    Mutation

    motor domain aa1-559

    Promoter

    chicken Beta Actin

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

    Plasmid

    #165429

    Purpose

    Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    FHS-paCaMKII
    

    Use

    AAV

    Tags

    FHS(Flag, HisX6, Strep) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII0.4

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG_EGFP-GFE3

    Plasmid

    #79869

    Purpose

    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CFAST-Halotag-ER

    Plasmid

    #214413

    Purpose

    ER localization of CFAST fused to Halotag-ER

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    rat cb5 ERM
    

    Use

    Tags

    CFAST-Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-Dynamin 2 K44A

    Plasmid

    #22301

    Depositor

    Pietro De Camilli

    Article

    Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

    Insert

    Dynamin 2 K44A (Dnm2 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pMRX-IP/Venus-rLC3

    Plasmid

    #58740

    Purpose

    Expresses Venus-rLC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

24. Cav2.1 HA pcDNA3

    Plasmid

    #206069

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBiFC-bFOSDelataZipVC155

    Plasmid

    #22014

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bFosDelataZIPVC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of residues 179-193 in bFos(118-211)

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. myc-mTOR (1750-2549 aa)

    Plasmid

    #21747

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1750-2549) (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1750-2549

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

27. K44A dynamin 2 pEGFP

    Plasmid

    #34687

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV ORANGE Dlg4-HaloTag KI

    Plasmid

    #139656

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX-T-rnVAPA-1-125

    Plasmid

    #13396

    Depositor

    Axel Brunger

    Article

    Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

    Insert

    VAP-A (Vapa Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pGEX-4T1-14-3-3 epsilon GST

    Plasmid

    #13279

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

    Insert

    14-3-3 epsilon (Ywhae Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge