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pISH-Meis1
Plasmid#74348PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Meis1. Not for gene expression.DepositorInsertMeis homeobox 1 (Meis1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOM20MTS-mCherry-EGFP-Tet-On
Plasmid#109016PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondriaDepositorInsertTOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MP9956:SFG.GD3synthase-2A-GD2synthase
Plasmid#75013PurposeRetroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.DepositorInsertsmurine GD3 synthase
murine GD2 synthase
UseRetroviral; Momlv retroviral vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag9
Plasmid#169335PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cellsDepositorInsertTOMM20-HaloTag9 (TOMM20 Human)
UseTagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-TOMM20-N-10
Plasmid#55449PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 433, Emission: 475DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP
Plasmid#169333PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cellsDepositorInsertTOMM20-HaloTag9 (TOMM20 Human)
UseTagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_TOM20
Plasmid#111678PurposeMAC-tagged gene expressionDepositorInsertTOM20 (TOMM20 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-IRES-tdTomato-FlagAkt2W80A
Plasmid#64834PurposecDNA of Akt2 with point mutation from Trp to Ala at position 80. This mutation confers resistance to MK-2206 drug.DepositorInsertAkt2 (Akt2 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation Trp 80 to AlaPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK
Plasmid#158616PurposeB6J Id1-LSL-DTA allele targeting vector, low copy numberDepositorInsertId1 (Id1 Mouse)
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationInsertion of IRES-loxP-NeoR-loxP-DTA after the st…PromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry N1 TOMM20
Plasmid#157761PurposeExpression of TOMM20:mcherry in cellsDepositorInsertTOMM20 (TOMM20 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-TOMM20
Plasmid#207789PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.DepositorInsertsgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Tom20-Halo7Tag
Plasmid#111135PurposeTom20 with selflabeling tag, e.g. for fluorescence microscopyDepositorInsertReceptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
UseTagsHalo7 TagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-GFP-TRIM28
Plasmid#185010PurposeEncodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFPDepositorInsertTRIM28 sgRNA (Trim28 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Clover-TOMM20-N-10
Plasmid#56307PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515DepositorInsertTOMM20 (TOMM20 Human)
UseTagsCloverExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby2-TOMM20-N-10
Plasmid#55913PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTFP1-TOMM20-N-10
Plasmid#55511PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 462, Emission: 492DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgDnmt1_1
Plasmid#138347PurposeExpress guide RNA 1 for Dnmt1DepositorInsertmouse Dnmt1 sgRNA (Dnmt1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgDnmt1_2
Plasmid#138348PurposeExpress guide RNA 2 for mouse Dnmt1DepositorInsertmouse Dnmt1 sgRNA (Dnmt1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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mIFP-TOMM20-N-10
Plasmid#56239PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 683, Emission: 704DepositorInsertNM_014765.2 (TOMM20 Human)
UseTagsmIFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEMS-Tom20-mEGFP-reHaloTagF
Plasmid#200190PurposeTom20 with reversible HaloTagF variant for fluorescent labelingDepositorInsertTom20 (TOMM20 Human)
UseTagsmEGFP and reHaloTagFExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-TOMM20
Plasmid#227307PurposeDonor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)DepositorInsertTOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/tom20-pbira(79-c)-ha
Plasmid#154456Purposeexpresses tom20-pbira(79-c)-ha/biochemical assayDepositorInserttom20-pbira(79-c)-ha (TOMM20 Human)
UseTagsExpressionMammalianMutationPromoterCMV-FAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FLAG-Akt2-CA
Plasmid#64050PurposeMyristoylated-FLAG-tagged Akt2, constitutively activeDepositorInsertAKT2 (AKT2 Human)
UseLentiviralTagsFLAG and MYRExpressionMammalianMutationPromoterUbiquitinAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Tom20-mEGFP-reHaloTagS
Plasmid#200189PurposeTom20 with reversible HaloTagS variant for fluorescent labelingDepositorInsertTom20 (TOMM20 Human)
UseTagsmEGFP and reHaloTagSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSico Dnmt1
Plasmid#12165DepositorInsertDnmt1 shRNA (Dnmt1 Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …PromoterAvailable sinceJune 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-TOMM20-mEGFP
Plasmid#115911Purposedonor to insert EGFP at the TOMM20 locus in human cellsDepositorInsertTOMM20-mEGFP (TOMM20 Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-TOMM20-dTomato
Plasmid#115914Purposedonor to insert dTomato at the TOMM20 locus in human cellsDepositorInsertTOMM20-dTomato (TOMM20 Human)
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-myr-HA-Akt1
Plasmid#46969Purposelentiviral expression of myr-HA-Akt1DepositorInsertAKT1 (AKT1 Human)
UseLentiviralTagsHA and myrExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt1
Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mNeon-Puro-TOMM20
Plasmid#207790PurposeDonor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)DepositorInsertTOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOM20*-SBP-GFP
Plasmid#120173PurposeExpresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)DepositorInsertMitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
UseTagsSBP and eGFPExpressionMammalianMutationTruncated TOM20: 1-30 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSicoR Dnmt1
Plasmid#12166DepositorInsertDnmt1 shRNA (Dnmt1 Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …PromoterAvailable sinceJune 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-mHdac1-L2
Plasmid#122307PurposeExpresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Hdac1 (Hdac1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt3
Plasmid#53596Purposelentiviral expression of constitutively active human Akt3 and EGFPDepositorInsertcAkt3 (AKT3 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-mmDNMT1
Plasmid#205635PurposereRNA guide targeting Mus Musculus DNMT1DepositorInsertreRNA-mmDNMT1 (Dnmt1 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-8:TOMM20-mEGFP
Plasmid#87423PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20DepositorInsertTOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits
