164,577 results

1. qTAG-N-Blast-ultraID

   Plasmid

   #207682

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA5 CMV 3xFG eIF4G1

   Plasmid

   #158775

   Purpose

   pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYFAC-CH2-ubi-M-pyrG

   Plasmid

   #221132

   Purpose

   Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

   Type

   Empty backbone

   Use

   Synthetic Biology; Expression in aspergillus and …

   Tags

   Expression

   Mutation

   Promoter

   PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ubi:Zebrabow

   Plasmid

   #118219

   Purpose

   For ubiquitous expression of Brainbow in zebrafish

   Depositor

   Albert Pan, Alex Schier

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_BiPVe3_dTomato_nlsdTomato

   Plasmid

   #213940

   Purpose

   AAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WP0003_dTomato_nlsdTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   dTomato_P2A_nls.dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-PH(Akt2)

   Plasmid

   #86596

   Purpose

   Expresses mCherry-tagged PH(WT) domain of human Akt2

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2 PH domain (AKT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1 - 108

   Promoter

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ10844

   Plasmid

   #183956

   Purpose

   CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEx-ER-TurboID

   Plasmid

   #160857

   Purpose

   Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304 YAP1

   Plasmid

   #193689

   Purpose

   Constitutive lentiviral expression of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. L4440

    Plasmid

    #1654

    Depositor

    Andrew Fire

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, T7p, lacZN, OriF1>>, OriF1<<

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. EZ-Tet-pLKO-Hygro

    Plasmid

    #85972

    Purpose

    Tet/Dox inducible shRNA lentivirus with hygromycin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1 / TetO

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

    Plasmid

    #98928

    Purpose

    Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    Ruby2-smFP-Flag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human Beta-catenin GFP

    Plasmid

    #71367

    Purpose

    mammalian expression of human Beta-catenin GFP

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    Beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSC-NLS-RFP

    Plasmid

    #221813

    Purpose

    Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NLS-mCherry
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ALK5 T204D

    Plasmid

    #80877

    Purpose

    mammalian expression of constitutively active ALK5 T204D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

    Insert

    ALK5 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (constitutively active)

    Promoter

    CMV

    Available Since

    Aug. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pEA186

    Plasmid

    #187874

    Purpose

    Empty pVS1-based INTEGRATE vector with mCherry cargo

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVE-eGFP

    Plasmid

    #52581

    Purpose

    Lentiviral transfer vector driving expression of eGFP in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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