Addgene

We narrowed to 26,385 results for: CAL

Showing: 6881 - 6920 of 26385 results

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mVermilion-C1

Plasmid

#105756

Purpose

Empty vector for creating N terminal mVermilion fusions

Depositor

Megan Rizzo

Article

Lee et al Elife. 2021 Oct 29;10. pii: 67858. doi:

Type

Empty backbone

Use

Tags

mVermilion

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-NS

Plasmid

#140874

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSuper-PICK1 shRNA

Plasmid

#67522

Purpose

Expresses shRNA against PICK1

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Mouse, Rat)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarletI

Plasmid

#201442

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-600n

Plasmid

#13541

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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p3E-csChBP

Plasmid

#108893

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

dChBP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

June 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Vinculin tail

Plasmid

#107154

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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USP16_pCSdest

Plasmid

#53878

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

USP16 (USP16 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
RBM11_pCSdest

Plasmid

#53861

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RBM11 (RBM11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDOC-K-glmS-lacIZ

Plasmid

#158061

Purpose

Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

lacIZ operon

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Aahn

Plasmid

#112158

Purpose

A genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.

Depositor

Bradley Baker

Article

Sepehri Rad et al Sci Rep. 2018 May 2;8(1):6911. doi: 10.1

Insert

Aahn

Use

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p4973 pcDNA4-E6AP III C21Y

Plasmid

#32890

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Use

Tags

6 His Express and T7-tag

Expression

Mammalian

Mutation

C21Y

Promoter

CMV

Available since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA HA FAK Y861F

Plasmid

#50508

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Use

Tags

HA

Expression

Mammalian

Mutation

Y861F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAT206/Sept7shRNA

Plasmid

#38299

Depositor

Matthew Krummel

Article

Tooley et al Nat Cell Biol. 2009 Jan;11(1):17-26. Epu

Insert

Septin 7 (Septin7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
pAM5089

Plasmid

#85125

Purpose

KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiB-kaiB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEYFP-Syx2

Plasmid

#35703

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx2 (Plekhg5 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pXJ40-Flag-iBox-PAK4cat

Plasmid

#190178

Purpose

expresses Flag-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

iBox-PAK4cat

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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MTK8b_005

Plasmid

#123893

Purpose

Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5' AAV ITR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_021

Plasmid

#123802

Purpose

Encodes VPR activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VPR AD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-3mNG

Plasmid

#179832

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-hygro-mScarletI

Plasmid

#179820

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-mCardinal

Plasmid

#179796

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCardinal

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔPQ

Plasmid

#35187

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 34-113

Promoter

CMV

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
pCB150-2_GFP-EB1

Plasmid

#46364

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

EB1 (MAPRE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

pBABE

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tH)

Plasmid

#109539

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Hras

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pF3BGX-T2A-GAL4

Plasmid

#138392

Purpose

RMCE vector for generating C-terminal T2A-GAL4 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-DJ1-WT

Plasmid

#29350

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
LDB107

Plasmid

#185423

Purpose

NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

Depositor

Laura Davis, Ken Belanger

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

NUP1

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_ROR2

Plasmid

#187759

Purpose

MAC-tagged gene expression of human ROR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROR2 (ROR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Mouse, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM394

Plasmid

#134211

Purpose

Baculovirus expression of CENP-N and CENP-L

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N-his-CENP-L

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pISceI-myl7:MBIC5-mCer3

Plasmid

#74117

Purpose

Zebrafish transgenic construct

Depositor

Michael Tsang

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Mrna generation for microinjection.

Tags

mCerulean 3

Expression

Mammalian

Mutation

Promoter

SP6

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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MAC_C_NTRK3

Plasmid

#187754

Purpose

MAC-tagged gene expression of human NTRK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

NTRK3 (NTRK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR1055

Plasmid

#71823

Purpose

Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-Paxillin-22

Plasmid

#55436

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Paxillin

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
Degron-KI ezh2 donor vector

Plasmid

#65620

Purpose

Donor vector to generate Degron-KI DD-EZH2

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

EZH2 left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
TDGS50

Plasmid

#183189

Purpose

TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

Depositor

Hsiao-Chun Huang

Article

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

DivIVA-GFP(tevR M0050)
TEV-S219V

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPglu-170nDelta13

Plasmid

#13562

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

WT binding protein (no F16A)

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG::PSAMQ79G:GlyR-IRES-GFP

Plasmid

#32482

Purpose

Expresses PSAM-GlyR (Q79G) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G-GlyR

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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