We narrowed to 17,014 results for: Por

1. pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

   Plasmid

   #205762

   Purpose

   Single-color FUCCI4 senor for transitions of all four cell cylce phases

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Miro1 S156E

   Plasmid

   #127589

   Purpose

   Phospho-mimetic of S156

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S156E

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4_10xUAS-MLPmin-luc2

   Plasmid

   #194383

   Purpose

   Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   10xUAS-luc2
   

   Expression

   Mammalian

   Promoter

   MLP minimal

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.M6PR-GFP

   Plasmid

   #224407

   Purpose

   Mammalian expression of M6PR

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   M6PR (M6PR Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Chicken Beta actin

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115219

   Purpose

   Expresses PspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PspCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. p2,0_3M5M_eGFP

   Plasmid

   #135474

   Purpose

   Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, eGFP reporter
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-gRNA

   Plasmid

   #158973

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   gRNA3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

   Plasmid

   #164612

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Expression

   Mammalian

   Mutation

   S383T, F177A

   Promoter

   CMV

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLNCX2-mEGFP-VPS4A

    Plasmid

    #116924

    Purpose

    expresses mEGFP-VPS4A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    silent mutation in position 4059 C to T

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pyronic/pcDNA3.1(-)

    Plasmid

    #51308

    Purpose

    Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

    Insert

    Pyronic
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215327

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    MUC5AC Promoter
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    MUC5AC

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBbsr-JNK KTR-mCherry

    Plasmid

    #115493

    Purpose

    Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

    Insert

    JNK KTR-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry G229L, mCherry Y298 deleted

    Promoter

    CAG

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-VEGFR2-mCherry

    Plasmid

    #139606

    Purpose

    Expresses a VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24a-VHH-mCherry

    Plasmid

    #109421

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. OMM-V5-LOV-Turbo

    Plasmid

    #199665

    Purpose

    expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    AKAP1 transmembrane domain and V5

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

    Plasmid

    #174876

    Purpose

    PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    RNAPII Ser2ph mintbody-SNAP
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-p58

    Plasmid

    #166942

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    p58 (Lman1 Rat)
    

    Tags

    GFP and chicken lysozyme signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. h-Plekhm1_EGFP

    Plasmid

    #73836

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

    Plasmid

    #205447

    Purpose

    human AAVS1-targeting plasmid for expression of mouse CR1

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCR1
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115267

    Purpose

    Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    LbuCas13a
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-JNK1

    Plasmid

    #86830

    Purpose

    mammalian expression of GFP-JNK1

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    JNK1 (MAPK8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Expression

    Yeast

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMX-eSOX17FNV

    Plasmid

    #118838

    Purpose

    Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX17FNV (Sox17 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    FNV: L46F, V53N, E57V

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Oct4-IRES-eGFP-PGK-Neo

    Plasmid

    #48681

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    CRISPR

    Tags

    GFP

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXS-IRES-Blast SLC5A7

    Plasmid

    #218539

    Purpose

    human SLC5A7 cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    SLC5A7 (SLC5A7 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXS-IRES-Blast SLC44A1

    Plasmid

    #218538

    Purpose

    human SLC44A1 cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    SLC44A1 (SLC44A1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. phASCL1-N106

    Plasmid

    #31781

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    achaete-scute complex homolog 1 (ASCL1 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV-FlicR1

    Plasmid

    #74142

    Purpose

    Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194693

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194695

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTACR TH1-p2aGFP

    Plasmid

    #135815

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 1 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTACR TH2-p2aGFP

    Plasmid

    #135816

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 2 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p2,0_3M5M_luciferase

    Plasmid

    #135475

    Purpose

    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, luciferase reporter
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDisplay-GRAB_OT1.0

    Plasmid

    #185384

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hsyn-GRAB_Ado1.0med

    Plasmid

    #153286

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Cre-responsive Cas9 expressing transposon library vector

    Plasmid

    #164138

    Purpose

    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, EF-1a

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

    Plasmid

    #188391

    Purpose

    Reporter for BCAM expression

    Depositor

    Markus Frank

    Article

    Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

    Insert

    BCAM reporter
    

    Use

    Luciferase

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAVTRE3_tdTomato

    Plasmid

    #135178

    Purpose

    AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

    Inserts

    tdTomato
    TRE3G
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    a variant of DsRed

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge