We narrowed to 7,519 results for: Lif

1. pBMN-AS-YY1

   Plasmid

   #154943

   Purpose

   Knocking down of human YY1 through shRNA and amiRNA targeting YY1

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   shRNA and amiRNA against YY1 (YY1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ins:GCaMP6s; cryaa:RFP

   Plasmid

   #110285

   Purpose

   A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Insert

   GCaMP6s
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   insulin

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-N-mEGFP-PARP11

   Plasmid

   #177998

   Purpose

   Expresses N-terminally mEGFP-tagged PARP11

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP11 (PARP11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE-mClover3-MAP4-MTBD

   Plasmid

   #171498

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   So et al Science. 2019 Jun 28;364(6447). pii: 364

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBbleo-ErbB2

   Plasmid

   #197361

   Purpose

   Encoding human ErbB2.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBabe puro-eIF4E

   Plasmid

   #33252

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   eIF4E (EIF4E Human)
   

   Use

   Retroviral

   Tags

   untagged

   Expression

   Mammalian

   Mutation

   unmodified

   Promoter

   SV40

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pENTR1A mKate2-hu_ncOGT K842M

   Plasmid

   #154284

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   K842M mutation (Catalytic Inactive)

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Puro-TDP-43-P112H

   Plasmid

   #133760

   Purpose

   Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed proline 112 to histidine.

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-Puro-TDP-43-A90V

   Plasmid

   #133754

   Purpose

   Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed alanine 90 to valine

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. P119 C-Check

    Plasmid

    #66817

    Purpose

    Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

    Depositor

    Yonglun Luo

    Article

    Zhou et al Cell Mol Life Sci. 2016 Jan 11.

    Insert

    truncated Enhanced Green Fluorescent Protein and asRED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

    Plasmid

    #205098

    Purpose

    Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

    Insert

    mNeonGreen, eKL9h, and Volvox channelrhodopsin-1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsHelz_AG

    Plasmid

    #148799

    Purpose

    Mammalian Expression of HsHelz

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz (HELZ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL11055

    Plasmid

    #68252

    Purpose

    Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro-HA-PIK3CA(H1047R;I848V)

    Plasmid

    #33253

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and isoleucine…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

15. anxA10-GFP

    Plasmid

    #107199

    Purpose

    Expresses human annexin A10-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

    Insert

    annexin A10 (ANXA10 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-Puro-TDP-43-D169G

    Plasmid

    #133757

    Purpose

    Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed aspartate 169 to glycine.

    Promoter

    CMV

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR1A mKate2-hu_ncOGT D554N

    Plasmid

    #154283

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    D554N mutation (Ser/Thr Glycosylation Impaired)

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScalps_Puro_mCebpe

    Plasmid

    #79076

    Purpose

    Expression of mouse Cebpe

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp epsilon (Cebpe Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid

    #102986

    Purpose

    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPBpuro-ErbB1(DEL1011)

    Plasmid

    #197366

    Purpose

    Encoding human ErbB1 mutant.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant. (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 1011-1186

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLPC-Flag-CHES1L

    Plasmid

    #51836

    Purpose

    encodes the long isoform of human CHES1 and a Flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

22. 139H2_LC

    Plasmid

    #206202

    Purpose

    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBabe puro-HA-PIK3CA(H1047R;G320E)

    Plasmid

    #33254

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and glycine 32…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pMSCV-PIG-Lin28b

    Plasmid

    #64795

    Purpose

    Expresses human Lin28b in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Lin28b (LIN28B Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBabe-HA-Chk2-D311V

    Plasmid

    #41921

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    Chk2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D311V

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pDual-eGFP(H66)

    Plasmid

    #63558

    Purpose

    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(H66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pBW_0001

    Plasmid

    #102833

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR221_SLC7A3

    Plasmid

    #132301

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

    Insert

    SLC7A3 (SLC7A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

    Plasmid

    #127790

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFLAG-CMV2 MAML LHHLL

    Plasmid

    #87681

    Purpose

    Mammalian expression vector for Maml1 with LHHLL mutation

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    MAML1 (MAML1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Mutate 1010,1011 from DD to HH

    Promoter

    CMV

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge