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We narrowed to 8,371 results for: BLI;

Showing: 6921 - 6960 of 8371 results

  1. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #219653
    Purpose
    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus
    Depositor
    Thomas Oertner
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)
    Expression
    Mammalian
    Mutation
    Promoter
    nEf Addgene # 149296
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    219653-plasmid-map-sequence-id-434847

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  2. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  3. MECP2 - S411R in pENTR

    Plasmid
    #216603
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    S411R
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216603-plasmid-map-sequence-id-427161

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  4. MECP2 - P388S in pENTR

    Plasmid
    #216602
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    P388S
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216602-plasmid-map-sequence-id-427158

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  5. MECP2 - P376S in pENTR

    Plasmid
    #216601
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    P376S
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216601-plasmid-map-sequence-id-427157

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  6. MECP2 - E282G in pENTR

    Plasmid
    #216600
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    E282G
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216600-plasmid-map-sequence-id-427156

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  7. MECP2 - P217L in pENTR

    Plasmid
    #216599
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    P217L
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216599-plasmid-map-sequence-id-426938

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  8. MECP2 - A202G in pENTR

    Plasmid
    #216598
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    A202G
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216598-plasmid-map-sequence-id-426912

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  9. MECP2 - G195S in pENTR

    Plasmid
    #216597
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    G195S
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216597-plasmid-map-sequence-id-426908

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  10. MECP2 - P152L in pENTR

    Plasmid
    #216596
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    P152L
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216596-plasmid-map-sequence-id-426904

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  11. MECP2 - P152R in pENTR

    Plasmid
    #216595
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    P152R
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216595-plasmid-map-sequence-id-426901

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  12. MECP2 - R91W in pENTR

    Plasmid
    #216593
    Purpose
    gateway cloning donor vector containing MECP2 ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    R91W
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216593-plasmid-map-sequence-id-426897

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  13. pET21b-hHNF4aORF-1

    Plasmid
    #206959
    Purpose
    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206959-plasmid-map-sequence-id-422372

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  14. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208979-plasmid-map-sequence-id-418375

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  15. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

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  16. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  17. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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  18. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208647-plasmid-map-sequence-id-418374

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  19. pUAS-kkv-OH

    Plasmid
    #138956
    Purpose
    Used to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv with C terminal epitope tag (kkv Fly)
    Use
    Tags
    ollas and his-6 added to C terminus of protein co…
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138956-plasmid-map-sequence-id-274764

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  20. pHD-DsRed-kkv-3::NG

    Plasmid
    #138960
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    3' end of kkv and neon green (kkv Fly)
    Use
    An editing vector for drosophila
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138960-plasmid-map-sequence-id-274768

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  21. pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

    Plasmid
    #201187
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 2 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201187-plasmid-map-sequence-id-398106

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  22. pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

    Plasmid
    #201188
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 3 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201188-plasmid-map-sequence-id-398098

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  24. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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  25. mouse KANK4-Y801H Ankyrin Repeats pET151

    Plasmid
    #164058
    Purpose
    Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164058-plasmid-map-sequence-id-327667

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  28. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid
    #140458
    Purpose
    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Halo, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140458-plasmid-map-sequence-id-292298

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  29. CD44I-c014

    Plasmid
    #175451
    Purpose
    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    AviTag-His6
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175451-plasmid-map-sequence-id-353223

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  30. CD44U-c011

    Plasmid
    #175454
    Purpose
    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    AviTag-His6
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175454-plasmid-map-sequence-id-353220

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  31. CD44I-c011

    Plasmid
    #175449
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175449-plasmid-map-sequence-id-353150

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  36. pB6-Id1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid
    #139279
    Purpose
    B6J Id1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    C-terminal fusion of T2A-sfGFP-iCre-ERT2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    139279-plasmid-map-sequence-id-315358

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  38. pFP.R373

    Plasmid
    #138243
    Purpose
    Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GKA).SaP-dpol*.(CEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    July 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    138243-plasmid-map-sequence-id-304854

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  39. pFP.R362

    Plasmid
    #138232
    Purpose
    Expresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(ASC).CbP-RNR*.(CRL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138232-plasmid-map-sequence-id-296589

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  40. pFP.R376

    Plasmid
    #138246
    Purpose
    Expresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGG).Pfu-RIR1-1*.(TGL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138246-plasmid-map-sequence-id-296588

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Showing: 6921 - 6960 of 8371 results