We narrowed to 5,300 results for: pcDNA3.1+

1. pFH2.21_mCherry-MS2x6

   Plasmid

   #205536

   Purpose

   Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-PH

   Plasmid

   #96948

   Purpose

   EGFP-PLC pleckstrin homology domain for CALI validation

   Depositor

   Marcel Bruchez

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   Pleckstrin homology domain
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-EcPPK1

   Plasmid

   #159279

   Purpose

   Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

   Insert

   HA-tagged Polyphosphate Kinase 1
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIR-FbGFPY58/C-JIP

   Plasmid

   #184684

   Purpose

   NIR-Fb-based JNK inhibitor

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbGFPY58/C-JIP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hGFAP R239C

   Plasmid

   #169476

   Purpose

   Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   EGFP-hGFAP (GFAP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed R 239 to C

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. perbB2K732M-ECFP

   Plasmid

   #40269

   Depositor

   Martin Offterdinger

   Article

   Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

   Insert

   erbB2 (ERBB2 Human)
   

   Use

   Tags

   ecfp

   Expression

   Mammalian

   Mutation

   K732M

   Promoter

   CMV

   Available since

   Jan. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

   Plasmid

   #169534

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven miochondria-localized yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-optoB2-SS-YFP

   Plasmid

   #75270

   Purpose

   Arrestin independent version of optoB2 with serine mutations at S362A,S363G

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   Arrestin independent version of optoB2
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   serine mutations at S362A,S363G

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 SWS cone opsin

   Plasmid

   #109363

   Purpose

   Human SWS cone opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   SWS cone opsin (OPN1SW Human)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBT140

    Plasmid

    #27493

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

    Insert

    Nuclear localization signal-Cre
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mc2 neo EF1a

    Plasmid

    #206220

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BLING 1

    Plasmid

    #171647

    Purpose

    Expresses bioluminescent glutamate indicator in mammalian cells

    Depositor

    Assaf Gilad

    Article

    Petersen et al ACS Synth Biol. 2023 Jul 14. doi: 10.102

    Insert

    BLING 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP

    Plasmid

    #205531

    Purpose

    Express HIV MiniGagZip-MCP_Zip-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    MiniGagZip-MCP_Zip
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lmajor_gp63_10_0460_E265A

    Plasmid

    #171641

    Purpose

    Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

    Insert

    LmjF.10.0460 E265A
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    E265A

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus wGBD

    Plasmid

    #85222

    Purpose

    Venus tagged Cdc-42 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    wGBD (WASL Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Cdc42 binding domain of N-WASP

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HCMVgH (Merlin)

    Plasmid

    #136448

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMM585-Tier1-PhCMV-Igk

    Plasmid

    #169486

    Purpose

    Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Igk secretion signal (IGK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 3xFRB-myc

    Plasmid

    #20226

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-NBid-PhoCl1-CBid

    Plasmid

    #164050

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl1

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl1-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eTC GFP ß-actin ΔZip

    Plasmid

    #27124

    Depositor

    Robert Singer

    Article

    Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

    Insert

    beta-actin (ACTB Human)
    

    Use

    Tags

    EGFP and TC tag

    Expression

    Mammalian

    Mutation

    This construct lacks β-actin 3′UTR and therefore …

    Promoter

    Available since

    Jan. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge