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We narrowed to 31,489 results for: REP

Showing: 3501 - 3520 of 31489 results
  1. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    Insert
    mCherry-pHluorin
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Promoter
    pDAB
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Bacterial
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 3501 - 3520 of 31489 results