Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,780 results for: construct

Clear keywords
Showing: 7121 - 7160 of 8780 results

  5. TAAR8-DuET

    Plasmid
    #213383
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TAAR8 (TAAR8 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213383-plasmid-map-sequence-id-430738

    Enlarge

  6. TAAR9-DuET

    Plasmid
    #213384
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TAAR9 (TAAR9 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213384-plasmid-map-sequence-id-430739

    Enlarge

  10. CHRM1-DuET

    Plasmid
    #213207
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM1 (CHRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213207-plasmid-map-sequence-id-430894

    Enlarge

  12. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα-Smad7-SOCS1

    Plasmid
    #242412
    Purpose
    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitors, srIκBα, Smad7, and SOCS1.
    Depositor
    Ewan St. John Smith
    Article
    Lim et al bioRxiv 2024.09.24.614636
    Inserts
    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-srIκBα-Smad7-SOCS1
    Use
    Mouse Targeting; Self-amplifying rna
    Expression
    Mammalian
    Available Since
    Sept. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242412-plasmid-map-sequence-id-487191

    Enlarge

  14. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102890
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102890-plasmid-map-sequence-id-198249

    Enlarge

  17. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid
    #234869
    Purpose
    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.
    Depositor
    Michael Murrell
    Article
    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:
    Insert
    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    Use
    Lentiviral
    Tags
    GFP, RFP, FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    234869-plasmid-map-sequence-id-471233

    Enlarge

  18. pN251

    Plasmid
    #188471
    Purpose
    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)
    Depositor
    Le Cong
    Article
    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2
    Insert
    enCas12a-HF (gift from Dr. Keith Joung)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    188471-plasmid-map-sequence-id-370973

    Enlarge

  19. pHDR-eGFP (pXK-1647)

    Plasmid
    #208824
    Purpose
    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208824-plasmid-map-sequence-id-417244

    Enlarge

  21. palm-Bassoon-GFP

    Plasmid
    #250161
    Purpose
    Encodes a version of rat Bassoon with a C-terminal GFP-tag and with a palmitoylation consensus replacing the N-terminal 94 aa. For mammalian expression.
    Depositor
    Thomas Dresbach
    Article
    Kapoor et al Elife. 2026 Jan 13;13:RP98254. doi: 10.7
    Insert
    palmitoylated Bassoon-GFP construct (N-terminal 94 aa replaced by palmitoylation consensus) (Bsn Rat)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    250161-plasmid-map-sequence-id-498360

    Enlarge

  22. p5E-mpeg1.1

    Plasmid
    #75023
    Purpose
    Can be used to drive expression in zebrafish microglia.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    mpeg1.1
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75023-plasmid-map-sequence-id-141914

    Enlarge

  26. ADRB3-DuET

    Plasmid
    #213182
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRB3 (ADRB3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213182-plasmid-map-sequence-id-430749

    Enlarge

  27. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

    Enlarge

  28. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid
    #107292
    Purpose
    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Dmytro Yushchenko
    Article
    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191
    Insert
    SNCA-GFP (SNCA Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107292-plasmid-map-sequence-id-209472

    Enlarge

  30. pUCmini-iCAP-AAV9.452sub.LUNG1

    Plasmid
    #184592
    Purpose
    non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Mol Ther Methods Clin Dev. 2022 Aug 1;26
    Insert
    Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
    Use
    AAV
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available Since
    July 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    184592-plasmid-map-sequence-id-364433

    Enlarge

  31. PTGDR-DuET

    Plasmid
    #213369
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    PTGDR (PTGDR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213369-plasmid-map-sequence-id-430728

    Enlarge

  40. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

    Enlarge

Showing: 7121 - 7160 of 8780 results