-
Plasmid#91761PurposeBacterial expression of a fluorescent protein, CitrineDepositorInsertCitrine
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFPmG1
Plasmid#50444PurposeExpression of a EGFP variant in mammalian cellsDepositorInsertGFPmG1
UseTagsExpressionMammalianMutationE143K, N147QPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyTagsExpressionMutationG65S, L68V, A72S, Y203T, L231HPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_proB_eGFP
Plasmid#59845PurposeConstitutively expresses eGFP with proB promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproBAvailable sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT264
Plasmid#27438DepositorInsertTRE-tdTomato-myc flanked by insulators
UseMouse Targeting; Tta dependentTagsMycExpressionMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
Gq-sensor[mTq2-sREACh]
Plasmid#98835PurposeA FRET sensor for Gq activation, encoded on a single plasmid, for FLIMDepositorInsertmTq2-sREACh
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G
Plasmid#47646PurposepAC-EsaR with a a272g mutation in esaRDepositorInsertesaR-D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to GlycinePromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS-PluxIlux
Plasmid#47641Purposewt luxI promoter in pCS26DepositorInsertPluxI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPluxIAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-URA3-3'SEC13-GFP
Plasmid#25445DepositorInsertSec13-Green Fluorescent Protein
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTCW007
Plasmid#83525Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha1
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK1_eGFP
Plasmid#59853PurposeConstitutively expresses eGFP with proK1 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK1Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gq-sensor[mTq2-mNG]
Plasmid#98884PurposeA FRET sensor for Gq activation, encoded on a single plasmid.DepositorInsertmTq2-mNG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExo1
Plasmid#53357PurposeExpresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.DepositorInsertsBlaMs
hlyAs
UseTagsExpressionMutationPromoterp(lac/ara-1)Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-BFP
Plasmid#214363PurposeExpression of the Blue Fluorescent ProteinDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-V220A
Plasmid#47645PurposepAC-EsaR with a c659t mutation in esaRDepositorInsertesaR-V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Valine 220 to AlaninePromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFU-no toxin-PE
Plasmid#24149DepositorInsertPDGF-receptor-transmembrane domain (PDGFRB Human, Aequorea victoria)
UseLentiviralTagsEGFP and FLAGExpressionMammalianMutationPromoterAvailable sinceApril 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLBC0030
Plasmid#172777PurposeLevel 0 module containing NT2_nYFPDepositorInsertCDS N-terminal eYFP (1-174)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0031
Plasmid#172778PurposeLevel 0 module containing CT_nYFPDepositorInsertCDS N-terminal eYFP (1-174)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0040
Plasmid#172779PurposeLevel 0 module containing NT2_cYFPDepositorInsertCDS C-terminal eYFP (175-238)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0041
Plasmid#172780PurposeLevel 0 module containing CT_cYFPDepositorInsertCDS C-terminal eYFP (175-238)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux LasI
Plasmid#193628PurposeInducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterLux promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Clux
Plasmid#47658PurposePesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)DepositorInsertPesaR-C
UseSynthetic BiologyTagsExpressionBacterialMutationAdditional esa box between -35 site and the origi…PromoterPesaR-CAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-EGFP-9
Plasmid#197733PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBC0050
Plasmid#172781PurposeLevel 0 module containing NT2_cYFPDepositorInsertCDS 3XFLAG tag N-terminal eYFP (1-174)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0051
Plasmid#172782PurposeLevel 0 module containing CT_3XFLAGnYFPDepositorInsertCDS 3XFLAG tag N-terminal eYFP (1-174)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCW008
Plasmid#83526Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha2
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTNT-pT7-tetR-sfGFP
Plasmid#140868PurposeT7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)DepositorInsertTetR-sfGFP expressed with T7 promoter
UseSynthetic BiologyTagssfGFPExpressionBacterialMutationPromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V
Plasmid#47663PurposepAC-EsaR with a208g mutation in esaRDepositorInsertesaR-I70V
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to ValinePromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDTnLAPP2A frame 0
Plasmid#28226DepositorInsertHygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
UseRetroviralTagsEGFP and nLAP-tagExpressionMutationPromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAGH23
Plasmid#107262Purposecontains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.DepositorInsertcurT (slr0483 )
UseSynthetic BiologyTagsmTurquoise2ExpressionBacterialMutationPromoternative CurT promoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK6_eGFP
Plasmid#59850PurposeConstitutively expresses eGFP with proK6 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK6Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAP639-2F
Plasmid#99486Purpose3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlagDepositorInsert3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDTnLAPP2A frame 2
Plasmid#28228DepositorInsertHygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
UseRetroviralTagsEGFP and nLAP-tagExpressionMutationPromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDTnLAPP2A frame 1
Plasmid#28227DepositorInsertHygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
UseRetroviralTagsEGFP and nLAP-tagExpressionMutationPromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS303:VC
Plasmid#37556DepositorInsertVenus C-term (aa 155-238)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJK_pro6_eGFP
Plasmid#59851PurposeConstitutively expresses eGFP with pro6 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterpro6Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK14_eGFP
Plasmid#59848PurposeConstitutively expresses eGFP with proK14 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK14Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME_G_E_007_UAV_sfGFP_PaqCI
Plasmid#235975PurposeUniversal acceptor vector with sfGFP placeholder PaqCI compatible for lvl0 assemblyDepositorInsertUAV_sfGFP_PaqCI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_UAV_sfGFP
Plasmid#235973PurposeUniversal acceptor vector with sfGFP placeholder for lvl0 assemblyDepositorInsertUAV_sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_5_001_3'UTR_Placeholder
Plasmid#235905PurposeEncodes a sfGFP cassette in the 3'UTR position used for placeholder cloningDepositorInsert3'UTR placeholder
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only