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Plasmid#140306PurposeExpresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FLExpressionMammalianMutationcontains only the SH4 and UD regions fused to mCh…PromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CRISPR
Plasmid#160047PurposeFor cloning sgRNA to knockout a specific gene in piggybac plasmidDepositorTypeEmpty backboneUseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Incoherent feedforward (IFF) plasmid
Plasmid#169748PurposeStandalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.DepositorInsertmiR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
TAPBPL pmRFP-N2 (721)
Plasmid#62000Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTAPBPL (TAPBPL Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema6b(L)-AP-His
Plasmid#72039PurposeExpresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6b (Sema6b Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6c-AP-His
Plasmid#72041PurposeExpresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6c (Sema6c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM585
Plasmid#68884PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-L3-SpCas9-HF1-2A-EGFP-L2
Plasmid#141048PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF1-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.22-PGK1-HRE::dLUC
Plasmid#128095PurposeHypoxia response element real-time luciferase reporterDepositorInsertHypoxia response element (x3) from PGK1 promoter (PGK1 Human)
UseLuciferaseTagsdrives expression of destabilized luciferase (2CP…ExpressionMammalianMutationPromoterinserted PGK1 hypoxia response elements (HRE) (x3)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GFP-AURKA_K162M-mCherry
Plasmid#99881PurposeExpression of GFP-AURKA K162M-mCherry for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFP and mCherryExpressionBacterialMutationK162M (kinase-dead)PromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sema6b(S)-AP-His
Plasmid#72040PurposeExpresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6b (Sema6b Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-CD151-7
Plasmid#55282PurposeLocalization: Tetraspanin/Membrane, Excitation: 399, Emission: 456DepositorInsertCD151 (CD151 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-CMV-GCaMP6m
Plasmid#40754PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6m
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd12_pegRNA_(PP7-C4-Q1)
Plasmid#232435PurposepegRNA with optimized 3' modifications to correct the rd12 mutationDepositorInsert3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd12_nsgRNA(PP7)
Plasmid#232436PurposensgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloopDepositorInsertPP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV Ef1a DIO GeNL_SS hGtACR1
Plasmid#233511PurposeLuciferase opsin fusion protein for bioluminescent optogeneticsDepositorInsertsmNeonGreen
ISN-IE
hGtACR1
UseAAVTagsGaussia luciferase signal sequenceExpressionMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEYN12-mflon
Plasmid#165581PurposeLon protease from Mesoplasma florumDepositorInsertlon protease
UseTagsExpressionBacterialMutationPromoterPtrcAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-tdTomato-VPR
Plasmid#232632PurposeVPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacerDepositorInsertdCas9-tdTomato-VPR
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHW111101
Plasmid#222452PurposeLevel 1, Position 7 containing CBS4-miniDFR::Cre::NOS.DepositorInsertCBS4-miniDFR::Cre::NOS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdOm2.4
Plasmid#210281PurposeEmpty backbone with ampicillin resistance for low expression in mammalian cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)
Plasmid#219426PurposeCre-dependent expression of luciferase opsin fusion protein for bioluminescent optogeneticsDepositorInsertmNeonGreen, ISN-IE, ChRmine
UseAAVTagsGaussia luciferase signal sequenceExpressionMutationPromoterEF-1alphaAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB'_pET21b
Plasmid#215416PurposeExpression construct for NylB' gene, codon optimized for expression in E. coliDepositorInsertNylB'
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPER-DuET
Plasmid#213256PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPER (GPER1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DNAI1:1-699
Plasmid#211275PurposeMammalian expression of full-length human DNAI1. C-terminal HiBiT tag.DepositorInsertDNAI1:M1-T699 (DNAI1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationA8S Y202C P495Q annotated sequence conflictsPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)
Plasmid#193854PurposePromoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing padDepositorInsert5' Cbr-unc-119(+) + 5' mNeonGreen
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterCbr-unc-119pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAMCase_CatD_6xHis_pTwistCMV
Plasmid#200228PurposeExpresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vectorDepositorInsertAcidic Mammalian Chitinase (Chia1 Mouse)
UseTags6xHisExpressionMammalianMutationS354FPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only