161,709 results

1. pcDNA3-myc-CUL3

   Plasmid

   #19893

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. ADAMTS5_pCSdest

   Plasmid

   #53819

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   ADAMTS5 (ADAMTS5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCAS9-mCherry-Frame +2

   Plasmid

   #66941

   Purpose

   CRISPaint frame selector +2

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MRGPRD-DuET

   Plasmid

   #213340

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MRGPRD (MRGPRD Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twin-Strep(R)-Tev-GSGS-Ubiquitin

   Plasmid

   #186803

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   Twin-Strep(R)-Tev-GSGS-Ubiquitin
   

   Use

   Tags

   GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

   Expression

   Bacterial

   Mutation

   Strep tag Lysine's mutated to Arginine

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330S-2

   Plasmid

   #58778

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-GCaMP6s-WPRE-pGHpA

   Plasmid

   #67526

   Purpose

   "Fluorescent reporter for calcium imaging"

   Depositor

   Botond Roska

   Article

   Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. L4440_BioBrick-sgRNA

    Plasmid

    #177783

    Purpose

    Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-7 asyn WT

    Plasmid

    #36046

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-AsLOV2x3-NLSx3

    Plasmid

    #231566

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    dCas9-AsLOV2x3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. mycBioID2-13X Linker-MCS

    Plasmid

    #92308

    Purpose

    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Humanized A. aeolicus BioID2 with R40G and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. N1-Mito-pHCountdown

    Plasmid

    #182303

    Purpose

    mammalian cell plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    mitochondria targeted pH countdown
    

    Use

    Tags

    COX8A mitochondria targeting sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 MLK1

    Plasmid

    #60540

    Purpose

    Donor vector for human MLK1

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-EFS-MCS-WPRE

    Plasmid

    #163362

    Purpose

    The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbB5k-GFP

    Plasmid

    #35354

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CAG-FRT-Rev-3xGFP

    Plasmid

    #191204

    Purpose

    FlpO dependent GFP expression

    Depositor

    Janelia Viral Tools

    Article

    Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

    Insert

    3X GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207860

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HDM_Spikedelta21_sinobiological_B.1.617.2

    Plasmid

    #204149

    Purpose

    Expression plasmid for SARS-CoV-2 Delta variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 Delta variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-N1-FUS/TLS-FLAGC

    Plasmid

    #60362

    Purpose

    Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMApra-Ptac

    Plasmid

    #218357

    Purpose

    Empty vector with lacIq-Ptac expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mc2 mNeon

    Plasmid

    #206218

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

    Plasmid

    #207863

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LeGO-G

    Plasmid

    #27347

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLJM1-TMEM192-FLAG

    Plasmid

    #164977

    Purpose

    Lentiviral vector for TMEM192 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge