We narrowed to 19,203 results for: IRE

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pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-RG-mycStrep

Plasmid

#31840

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R95G

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pCRII-Sema4c-ISH-2kb

Plasmid

#68031

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4C (Sema4c Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Plasmid

#169370

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Use

CRISPR

Expression

Insect

Promoter

CPIJ039596

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC1-Rap2A-3xFLAG

Plasmid

#87972

Purpose

Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGREAT4

Plasmid

#170892

Purpose

GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pG10-90

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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ERAS

Plasmid

#55656

Purpose

expression of mouse ERAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

ERAS (Eras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
cmamxA1-GFP

Plasmid

#107195

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A1

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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GEM

Plasmid

#55680

Purpose

expression of mouse GEM

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

GEM (Gem Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
fgfprom luc

Plasmid

#69446

Purpose

basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Insert

minimal fgf4 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgShmt2_2

Plasmid

#106315

Purpose

Express Cas9 and sgRNA targeting mShmt2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting mShmt2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLPC myc-mRAP1 I312R

Plasmid

#73137

Purpose

Expression of myc-tagged mRAP1 with single point mutatoin I312R

Depositor

Agnel Sfeir

Article

Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

Insert

Rap1 (Terf2ip Mouse)

Tags

myc

Expression

Mammalian

Mutation

isoleucine changed to an arginine at amino acid 3…

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only
pRRG45-GluR1

Plasmid

#99070

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea GluR1 (Glutamate receptor 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

GluR1 (Glutamate receptor 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D1a(aa254-503)

Plasmid

#21520

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only
Pr2-deGFP-MGapt

Plasmid

#67736

Purpose

OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Pr1-deGFP-MGapt

Plasmid

#67735

Purpose

OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr1

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4d-ISH-2kb

Plasmid

#68032

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4D (Sema4d Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Plxnb1-ISH-2kb

Plasmid

#68035

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B1 (Plxnb1 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-Q50K-Y54M

Plasmid

#75264

Purpose

Expresses a double point mutant of human TTF-1

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

Q50K and Y54M in the homeodomain

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
pAc5.1A-Dm-miR-1

Plasmid

#22711

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 1 (mir-1 Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pUWR-Cad-mTFP

Plasmid

#58327

Purpose

Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-mTFP

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Cux2 in situ probe

Plasmid

#45623

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Cux2 in situ probe (Cux2 Mouse)

Use

In situ

Mutation

fragment contains nt 1069-1694 on NM_007804

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
TU#1806_CRISPR_unc-73_exon21

Plasmid

#82360

Purpose

to create unc-73E null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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p3x-Flag Gli3 37R3-2

Plasmid

#120305

Purpose

Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C

Depositor

David Liu

Article

Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

Insert

Intein 37R3-2, Gli3

Use

Synthetic Biology

Tags

3X Flag

Expression

Mammalian

Mutation

Contains mutations as reported in 2011 Chemistry …

Promoter

CMV

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D5(aa1670-1962)

Plasmid

#21519

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa1670-1962

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsXRN1_1174-1649_AH

Plasmid

#148903

Purpose

Mammalian Expression of HsXRN1_1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1_1174-1649 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)

Plasmid

#70194

Purpose

Derived from 21 with silent mutations to make BsaI, BbsI compatible

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-GW1D10(aa566-1343)

Plasmid

#21517

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa566-1343

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pIW069

Plasmid

#134133

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWay21-MKP-1CH2AB

Plasmid

#13475

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2AB (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-136

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pRRG260-Cyp1A1

Plasmid

#99081

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Cyp1A1 (also known as h.49.4f)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Cyp1A1 (also known as h.49.4f) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Hox A11b

Plasmid

#36466

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA11b (hoxa11b Zebrafish)

Use

Riboprobe synthesis

Promoter

n/a

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pOGG113

Plasmid

#133124

Purpose

pL0M-PU-NoP, promotorless region used as negative control

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

NoP promotorless region from plasmid pIJJ11268

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAF210

Plasmid

#52406

Purpose

Expresses Flag-tagged mouse Arf5

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf5 (Arf5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pJAF214

Plasmid

#52404

Purpose

Expresses Flag-tagged mouse Arf3

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf3 (Arf3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR sgSHMT2_2

Plasmid

#106310

Purpose

Express Cas9 and sgRNA targeting SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
M34 Xdsh PDZ bait-pGBT9

Plasmid

#17190

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Xdsh (dvl2.L Frog)

Tags

Gal4-DBD

Expression

Yeast

Mutation

Dsh bp 343-1191

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
T7T3-PacI-Neto1

Plasmid

#87672

Purpose

In situ probe for mouse Neto1

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Neto1 (Neto1 Mouse)

Expression

Bacterial

Mutation

partial sequence, (1244 to 2278bp of NM_144946.4)

Promoter

T7/T3

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Ubiquitin (1-76; L71Y)

Plasmid

#110756

Purpose

Bacterial expression of human Ubiquitin (1-76; L71Y)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

L71Y

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Plxnb3-ISH-2kb

Plasmid

#68037

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Sema4g-ISH-2kb

Plasmid

#68034

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4G (Sema4g Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-GW1D10(aa566-1343)

Plasmid

#21516

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GST

Expression

Mammalian

Mutation

contain aa566-1343

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
GST-TNR(aa1-204)

Plasmid

#21514

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GST

Expression

Mammalian

Mutation

contain aa1-204

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
GFP-GW1D1b(aa502-751)

Plasmid

#21521

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa502-751

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
XE160 XDshHA-pt mutant PDZ-CS2+

Plasmid

#16780

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Dishevelled (dvl2.L Frog)

Use

Xenopus expression

Tags

HA

Mutation

point mutation in the binding pocket of the PDZ b…

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
XE161 XDshHA-triple pt mutant-CS2+

Plasmid

#16779

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Dishevelled (dvl2.L Frog)

Use

Xenopus expression

Tags

HA

Mutation

triple point mutation outside of the PDZ binding …

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
5mCUC1 pBluescript

Plasmid

#12065

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Expression

Bacterial

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pNB788

Plasmid

#60165

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 19,203 results for: IRE