We narrowed to 17,639 results for: URE

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3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

Plasmid

#60492

Purpose

Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

3xnls-SGFP2(T65G)
SGFP2-3xNES

Tags

SGFP2 and SGFP2(T65G)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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SuExp-mPLD3

Plasmid

#201245

Purpose

express full length mouse PLD3 protein

Depositor

David Nemazee

Article

Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

Insert

PLD3 (Pld3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSI-359

Plasmid

#131131

Purpose

gRNA expression vector for A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

A-to-G reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-D53A-GFP

Plasmid

#208778

Purpose

MSCV vector expressing D53A mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Mutation

Changed aspartic acid 53 to alanine (D53A)

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag6-iCAM-1

Plasmid

#205210

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag6 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag18-iCAM-1

Plasmid

#205207

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag18 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag42-iCAM-1

Plasmid

#205205

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag42 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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(326) pAAV SV40-ZsGreen U6-gRNA

Plasmid

#163020

Purpose

Fluorescent reporter for Sa gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Zs Green

Use

AAV

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-tagBFP

Plasmid

#65266

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-Axin-IRES-GFP

Plasmid

#14716

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

Axin (Axin1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CALR

Plasmid

#67086

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CALR (CALR Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
p3E-ING2

Plasmid

#109554

Purpose

Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd8-1D4

Plasmid

#42270

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 8 (Fzd8 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

Plasmid

#46676

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-133a-1 (MIR133A1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLV.PARP1#5

Plasmid

#14548

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pHH0103_TNK2-1/1

Plasmid

#91377

Purpose

Protein expression and purification of human SH3 domain construct TNK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK2-1/1 (TNK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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SRSF9_2xRRM_pGEX

Plasmid

#135120

Purpose

Recombinant expression of SRSF9 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF9 (SRSF9 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-CCND1-HA-puro

Plasmid

#212670

Purpose

Express tagged cyclin D1

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Bin1b

Plasmid

#109509

Purpose

Muscle specific zebrafish expression of mKate2 fused to Bin1b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Bin1b (bin1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBLO2 casX

Plasmid

#123122

Purpose

E. coli expression vector for CasX sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX guide RNA

Use

CRISPR

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

Plasmid

#169531

Purpose

Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCool-hDDB1-BPB

Plasmid

#48639

Purpose

Expresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

hDDB1-BPB (DDB1 Human)

Expression

Bacterial

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
pBI-GFP-Bub1-deletion mutant

Plasmid

#16626

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 deletion mutant (BUB1 Human)

Expression

Mammalian

Mutation

Amino acids 1-75

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pLV.PARP1#4

Plasmid

#14547

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pAd-Track SIRT1 H355A

Plasmid

#8441

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
SEPTOPO

Plasmid

#64944

Purpose

source of SEP (Super Ecliptic pHluorin) sequence

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

SEP

Expression

Bacterial

Promoter

lac

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pTS2396-Tier1-PhCMV-4xCOX8_Ypet

Plasmid

#169534

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven miochondria-localized yellow fluorescent protein Ypet

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-FAPP1

Plasmid

#109503

Purpose

Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-FAPP1

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsMago_I

Plasmid

#146582

Purpose

Mammalian Expression of HsMago

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsMago (MAGOH Human)

Expression

Mammalian

Mutation

one non silent mutation Y123C compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

Plasmid

#32728

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Mutation

-947 AP-1 binding site TGAGTCA deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-2xFYVEhgs

Plasmid

#109505

Purpose

Muscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-2xFYVEhgs

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd2-1D4

Plasmid

#42264

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 2 (Fzd2 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TGFB1

Plasmid

#67080

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TGFB1 (TGFB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd4-1D4

Plasmid

#42266

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 4 (Fzd4 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-ULK1 (836-1050)-MBP

Plasmid

#203562

Purpose

Mammalian expression of ULK1 (836-1050) with MBP tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ULK1 (ULK1 Human)

Tags

MBP-10His

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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LbCas12a-DR-cr in PBCSK-

Plasmid

#126641

Purpose

Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Hoxb7 promoter

Plasmid

#21294

Depositor

Frank Costantini

Article

Srinivas et al Development. 1999 Apr . 126(7):1375-86.

Insert

Hoxb7 promoter (Hoxb7 Mouse)

Use

Promoter can be used to make transgenic mice

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-rab32a

Plasmid

#109621

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab32a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32a (rab32a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP-GFP-STX17TMΔC

Plasmid

#221737

Purpose

Stable expression of GFP-STX17TMΔC in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

STX17 (STX17 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-228 and 227-302

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

Plasmid

#216380

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
G1397 DddAtox-C–dSpCas9

Plasmid

#157836

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-rab14

Plasmid

#109600

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab14

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab14 (rab14 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0044 CjcCas7-11 full locus with synthetic array

Plasmid

#172502

Purpose

Encodes the CjcCas7-11 full locus for bacterial expression

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 full locus with synthetic array

Expression

Bacterial

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCNK58

Plasmid

#202461

Purpose

Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells

Depositor

Stephen Harrison, Arthur Palmer

Article

Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi

Insert

Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)

Expression

Bacterial

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
K401-iLid

Plasmid

#122484

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

Kinesin 1-401 iLid His tag

Tags

His6 and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Promoter

T7

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HM3C NaVAe1_p pET24b

Plasmid

#127854

Purpose

Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1p

Tags

3C Cleavage site, His, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-Flag-hsDicer (E1564A)

Plasmid

#41587

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

E1564A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
UKNP1.4.1

Plasmid

#97363

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-lamp1-mKate2

Plasmid

#109507

Purpose

Muscle specific zebrafish expression of mKate2 fused to lamp1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

lamp1 (lamp1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,639 results for: URE