163,606 results

1. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD_sfGFP3TCG4TAG

   Plasmid

   #174518

   Purpose

   Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   sfGFP
   

   Use

   Tags

   6 x His

   Expression

   Bacterial

   Mutation

   Recoded. TCG and TAG codons were introduced in po…

   Promoter

   araBAD

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmScarlet-i_C1

   Plasmid

   #85044

   Purpose

   Mammalian expression of mScarlet-i

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   mScarlet-i
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. 12XCSL-d1EGFP

   Plasmid

   #47684

   Purpose

   Notch reporter

   Depositor

   Urban Lendahl

   Article

   Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

   Insert

   TP-1 promoter
   

   Use

   Notch reporter construct

   Tags

   d1EGFP

   Expression

   Mutation

   hexamerized 50-bp EBNA2 response element of the T…

   Promoter

   NA

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROPseq-puro-v2

   Plasmid

   #127458

   Purpose

   Entry plasmid for guide cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-iSeroSnFR

   Plasmid

   #128484

   Purpose

   Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Insert

   iSeroSnFR
   

   Use

   Tags

   Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV CEPIA2mt

   Plasmid

   #58218

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA2mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V M339L

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYL156 (TRV RNA2)

   Plasmid

   #148969

   Purpose

   Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

   Insert

   Modified TRV RNA2
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   see comments section below

   Promoter

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor_Tn[himar1C9]

   Plasmid

   #213906

   Purpose

   Mariner transposon with ATc-inducible himar1C9 transposase

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TARDBP

    Plasmid

    #156073

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHM6-Q23

    Plasmid

    #40263

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Use

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSFFV_sfCherry2(1-10)

    Plasmid

    #82603

    Purpose

    Lentiviral vector for constitutive expression of sfCherry2(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    SFFV

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6M-PAK1 T423E

    Plasmid

    #12208

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Activated Pak1: T423E

    Promoter

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-GFP

    Plasmid

    #11154

    Purpose

    Mammalian expression vector for expression of GFP (EF1a promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EF1 alpha promoter (EEF1A1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. SunTagng22aa

    Plasmid

    #106438

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-PARP1

    Plasmid

    #211578

    Purpose

    CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJC5-3XHA-EGFP-PEX26

    Plasmid

    #139054

    Purpose

    HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

    Depositor

    David Sabatini

    Article

    Ray et al iScience. 2020 May 22;23(5):101109. doi:

    Insert

    3XHA-EGFP-PEX26
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    UBC

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGG-BC-TurboID-GOI

    Plasmid

    #209390

    Purpose

    Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGG-GOI-DE-TurboID

    Plasmid

    #209388

    Purpose

    Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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