161,547 results

1. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-12x

   Plasmid

   #61699

   Purpose

   Expresses PTDH mutant Opt12x

   Depositor

   Huimin Zhao

   Article

   Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

   Insert

   Opt12x
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pEYFP-N1-Pink1

   Plasmid

   #101874

   Purpose

   Transient expression of Pink1-YFP in mammalian cells

   Depositor

   Richard Youle

   Article

   Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

   Insert

   PTEN induced putative kinase 1 (PINK1 Human)
   

   Use

   Tags

   fluorescent tag EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-smURFP-T2A-mCardinal

   Plasmid

   #80348

   Purpose

   Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   mCardinal
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY1513 AAVS1 sgRNA

   Plasmid

   #234832

   Purpose

   sgRNA for AAVS1 STITCHR insertion

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   AAVS1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiV2-hU6-evopreQ1

   Plasmid

   #210189

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. BoxB in pcDNA3

   Plasmid

   #29727

   Depositor

   Howard Chang

   Article

   Wang et al Nature. 2011 Mar 20. ():.

   Insert

   5BoxB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG dCas9-KRAB-2A-EGFP

   Plasmid

   #92396

   Purpose

   CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9_KRAB_2A_EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRY2high-mCherry

   Plasmid

   #104063

   Purpose

   Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2high-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2PHR E490R

   Promoter

   CMV

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_pGK_LaG17_synNotch_Gal4VP64

    Plasmid

    #79127

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17_synNotch_Gal4DBDVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-21a_3xNLS_SpCas9_protein_expression

    Plasmid

    #114365

    Purpose

    Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

    Insert

    3xNLS_SpCas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK-Rbfox2-rABE-Flag

    Plasmid

    #191384

    Purpose

    Doxycycline inducible expression of Rbfox2-rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    Rbfox2-rABE (RBFOX2 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    tet-inducible promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEcCas-2.0

    Plasmid

    #190697

    Purpose

    Constitutive expressing of Cas9 and inducible expression of lambda Red

    Depositor

    Sheng Yang

    Article

    Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

    Insert

    Cas9 and Lamda Red system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE Flag HA ADAR1 p150

    Plasmid

    #237855

    Purpose

    Lentiviral expression of ADAR1 p150

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    ADAR1 (ADAR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE Flag HA ADAR1 p110

    Plasmid

    #237854

    Purpose

    Lentiviral vector expressing ADAR1 p110

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    ADAR1 (ADAR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDB_347

    Plasmid

    #231114

    Purpose

    CBE activity-based selection cloning vector

    Depositor

    David Root, John Doench

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

    Plasmid

    #188778

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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