159,557 results
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pcDNA3-HA-DNM2-R465W
Plasmid#155262PurposeMammalian expression of rat DNM2 fused to HADepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsHAExpressionMammalianMutationArginine 465 to TryptophanPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-Cas9
Plasmid#166601PurposeEncodes Cre-dependent Cas9 under control of the TREDepositorInsertCas9
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-3XKD
Plasmid#25049DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlyRS-His
Plasmid#124110PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV_LaG17_synNotch_TetRVP64
Plasmid#79128PurposeLentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)DepositorInsertLaG17 GFP nanobody synNotch TetRVP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorInsertCysteinyl-tRNA ligase (cysS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2069
Plasmid#141902PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB400
Plasmid#68665PurposeMCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-RAP2A-GFP
Plasmid#86124PurposeRetroviral vector expressing RAP2A-GFP-IRES-BsdDepositorInsertRAP2A (RAP2A Human)
UseRetroviralTags3xFLAG and GFPExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts-1A(sfGFP)
Plasmid#126975PurposeLevel 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationRepA A56V thermosensitive mutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB200
Plasmid#68591PurposeMCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSKBAD2
Plasmid#15335DepositorInsertDnaE C-intein
UseTagsGB1ExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
UseTagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ProRS-His
Plasmid#124117PurposeBacterial expression plasmid for PURE system componentsDepositorInsertProlyl-tRNA ligase (proS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TrpRS-His
Plasmid#124120PurposeBacterial expression plasmid for PURE system componentsDepositorInsertTryptophanyl-tRNA ligase (trpS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB1532B-RFP
Plasmid#101854PurposeGolden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheTagsExpressionMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tTA
Plasmid#149363PurposeAAV-mediated and Cre-dependent expression of tTA under the CAG promoter.DepositorInserttTA2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_ELF2
Plasmid#187737PurposeMAC-tagged gene expression of human ELF2DepositorInsertELF2 (ELF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneUseTagsmyc, HisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet-i_N1
Plasmid#85056PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorInsertLifeAct
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF2
Plasmid#155347PurposeExpress full-length human YTHDF2 with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)
Plasmid#231733PurposeProtein expression in insect cellsDepositorInsertsUseTags3C-Strep(II) and Flag-3CExpressionInsectMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DD-T6B-MUT-YFP
Plasmid#235144PurposeExpresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoterDepositorInsertDHFR-fused mutant T6B peptide
UseAAVTagsExpressionMutation5 tryptophan residues of T6B-WT mutatedPromoterhSynAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3KT9
Plasmid#234192PurposeHeterologous protein expression of aprataxin in Escherichia coliDepositorInsert3KT9 (APTX Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
AiP2107 - pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA
Plasmid#230553PurposeAiE2664m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX-dtA
Plasmid#232236PurposeExpresses diphtheria toxin A subunit in Cre-positive cells.DepositorInsertDiphtheria toxin A subunit
UseAAVTagsExpressionMammalianMutationPromoterCAAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-Flex-MCS-WPRE-SV40 - GCH14
Plasmid#230821PurposeAAV cloning vector for CRE dependent expression under the EF1a promoter.DepositorTypeEmpty backboneUseAAV and Cre/Lox; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDL1403
Plasmid#226635PurposeThis plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.DepositorInsertstfox
qstR
UseTagsExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct
Plasmid#212705Purposetargeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) systemDepositorInsertZFP143-FKBPV-2HA-P2A-GFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xPax7gRNA
Plasmid#224569PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.1t
Plasmid#210254PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCerulean
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
p205-5'-Switch-ON
Plasmid#217884PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218634PurposeProximity biotinylation of msNLRP3(ΔPYD, I125M-END)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationdelPYD (I125M-END)PromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTN-HaloTag_DCAF1:1-1507
Plasmid#210972PurposeMammalian expression of full-length human DCAF1. N-terminal HaloTag.DepositorInsertDCAF1:M1-E1507 (DCAF1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_DCAF1:1-1507
Plasmid#210973PurposeMammalian expression of full-length human DCAF1. C-terminal HaloTag.DepositorInsertDCAF1:M1-E1507 (DCAF1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEJS2195_Nme2-evoFERNY-i1
Plasmid#201528PurposeMammalian expression of domain inlaid Nme2-evoFERNY-i1DepositorInsertNme2-evoFERNY-i1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2197_Nme2-evoFERNY-i8
Plasmid#201530PurposeMammalian expression of domain inlaid Nme2-evoFERNY-i8DepositorInsertNme2-evoFERNY-i8
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2217_Nme2-rAPOBEC-nt
Plasmid#201523PurposeMammalian expression of N-terminally fused Nme2-rAPOBEC-ntDepositorInsertNme2-rAPOBEC-nt
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
