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pMLS338

Plasmid

#73719

Purpose

SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Unc-32::GFP(Cbr-unc-119)

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGL0_7 [pSa ORI]

Plasmid

#198923

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI

Use

Synthetic Biology

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBXCA3H

Plasmid

#47072

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hsGFP

Plasmid

#64912

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hNanogP8

Plasmid

#19776

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

NanogP8 (NANOGP8 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pRSET-B CFP

Plasmid

#108858

Purpose

Encodes for the fluorescent protein CFP

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

CFP

Expression

Bacterial

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pKB231

Plasmid

#170018

Purpose

Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Cecropin P1

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCA0.421

Plasmid

#203950

Purpose

Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-B YFP

Plasmid

#108856

Purpose

Encodes for the fluorescent protein enhanced YFP (eYFP)

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

eYFP

Expression

Bacterial

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pKB232

Plasmid

#170019

Purpose

Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CRAMP

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY2849

Plasmid

#74112

Purpose

Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG(Q103L)

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_chaining vector

Plasmid

#62394

Purpose

Empty Backbone

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBSDT-AII

Plasmid

#27179

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

MC1 promoter diphtheria toxin-A fragment

Use

Mouse Targeting

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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p350_LTJ_180bpCD90.1Template

Plasmid

#104988

Purpose

180bp HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

180bp genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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AgAK

Plasmid

#71290

Purpose

Expression of A. gambiae adenosine kinase

Depositor

Vern Schramm

Article

Cassera et al Biochemistry. 2011 Mar 22;50(11):1885-93

Insert

Adenosine Kinase

Tags

6X Histidine

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pAB202

Plasmid

#101674

Purpose

Opto-T7RNAP(563)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP(563)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCHW91003

Plasmid

#222449

Purpose

Level 1, Position 3 containing 35S::RUBY::OCS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

35S::RUBY::OCS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCI 9.0

Plasmid

#74229

Purpose

Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLucPEST

Depositor

James Inglese

Article

Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

Insert

3xFlag-FLuc-P2A-3xFlag-NLucPEST

Expression

Mammalian

Promoter

None

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-tetA-Ptet-Rtet

Plasmid

#189855

Purpose

Cre-inducible activation of tetA for tetracycline resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

Ptet-loxP-TT-loxP-Rtet-tetA

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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H2B-Strawberry

Plasmid

#20970

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Tags

Strawberry

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pET24b-BR1.0

Plasmid

#199435

Purpose

expresses BAmRed1.0 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMRed

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_62 [sfYFP]

Plasmid

#198974

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

sfYFP

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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A3RXB7

Plasmid

#177694

Purpose

expresses a N-acetyl hexosamine oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

N-acetylhexosamine oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAB203

Plasmid

#101675

Purpose

Opto-T7RNAP(563-F1)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP(563-F1)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EZ-L545

Plasmid

#173024

Purpose

Encodes OptoEXP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE ZO1myc

Plasmid

#30318

Depositor

Alan Fanning

Article

Fanning et al Mol Biol Cell. 2007 Mar . 18(3):721-31.

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

myc

Expression

Mammalian

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
EZ-L580

Plasmid

#173023

Purpose

Encodes OptoAMP4 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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