We narrowed to 23,118 results for: Sis

Showing: 9101 - 9150 of 23118 results

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p2T-CMV-BE4max-BlastR

Plasmid

#152991

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

U6

Available Since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-2uDelta13

Plasmid

#12990

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16F D152A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

D152A mutation in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-G418

Plasmid

#160018

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCB-CBEv1

Plasmid

#221138

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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3539 pMIG Bax

Plasmid

#8788

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bax (Bax Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pRU323

Plasmid

#167690

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-FLAG-mTUT4

Plasmid

#60041

Purpose

Mammalian expression of FLAG-tagged mouse TUT4

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT4 (Tut4 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221-NUDT5

Plasmid

#219954

Purpose

For gateway cloning of NUDT5

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

NUDT5 (NUDT5 Human)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MEIOC

Plasmid

#222562

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MEIOC (C17orf104 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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mt SSB

Plasmid

#205057

Purpose

SSB1 protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

SSB1 (SSB Human)

Expression

Bacterial

Mutation

WT

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z21

Plasmid

#120064

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

24xSunTag

Use

Pcr template

Tags

24xSunTag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRAB18::LUC

Plasmid

#141168

Purpose

Luminescent reporter for abscisic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtRAB18 promoter

Use

Luciferase

Expression

Plant

Promoter

AtRAB18, AT5G66400

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pUB6-VSV-GS

Plasmid

#190781

Purpose

Expresses VSV-GS in mammalian cells

Depositor

Jakob Reiser

Article

Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

Insert

VSV-GS

Tags

HA

Expression

Mammalian

Promoter

UbC

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRU292

Plasmid

#167687

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB3

Plasmid

#19471

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB3 (Dnajb3 Mouse)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag-HA-Merlin

Plasmid

#27104

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Tags

Flag and HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-P09

Plasmid

#120020

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET30 GLTP

Plasmid

#170733

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-GFP-PTEN-WT

Plasmid

#30391

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

GFP-PTEN-WT (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-WT

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
RSRF-Luc-2mut

Plasmid

#31819

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Expression

Mammalian

Mutation

two CTATATTTAG RSRF sites mutated to CGATATTTCG

Promoter

mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-DMC1

Plasmid

#222541

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMC1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMC1 (DMC1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUG-UBA1

Plasmid

#104665

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-GI-ZFP2

Plasmid

#42215

Purpose

Expresses GI-ZFP2 for light-inducible activation of gene expression

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

GIGANTEA-FLAG-ZFP2 (GI Mustard Weed, Synthetic)

Tags

FLAG

Promoter

CMV

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-attBr-ZsGreen-attP

Plasmid

#51265

Purpose

Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

attBr-ZsGreen-attP

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

Plasmid

#147924

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

6xbsMS2-Malat (Malat1 Mouse)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer3

Plasmid

#14778

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pFLIPglu-600uDelta13

Plasmid

#12991

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pJP118

Plasmid

#176494

Purpose

Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Insert

Lifeact peptide

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SHC003BSD

Plasmid

#133300

Purpose

The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

Depositor

Jingshi Shen

Article

Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU61

Plasmid

#167692

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z20

Plasmid

#120063

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-mTUT7

Plasmid

#60044

Purpose

Mammalian expression of FLAG-tagged mouse TUT7

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT7 (Zcchc6 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pET-CV-Ykt6

Plasmid

#168030

Purpose

Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Ykt6(Q131-K190)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Dapp1-PH pET11a

Plasmid

#71376

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Dapp1-PH (DAPP1 Human)

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 148-273

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
Gma-pCAMBIA-1301-STTM171

Plasmid

#84080

Purpose

Disturb the expression of miR171 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-GpNLuc_SV40:PuroR

Plasmid

#183037

Purpose

Expresses the FLAG-tagged GpNLuc (Green LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR L1-vas2-Cre-L4

Plasmid

#62301

Purpose

Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mOPN-PB0

Plasmid

#106449

Purpose

Bacterial expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

WT

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAA5::LUC

Plasmid

#141169

Purpose

Luminescent reporter for auxin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtIAA5 promoter

Use

Luciferase

Expression

Plant

Promoter

AtIAA5, At1g15580

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6627

Plasmid

#106159

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRTP801/TA-ODD

Plasmid

#206212

Purpose

An expression vector of hypoxia-responsive transactivator under control of RTP801 promoter

Depositor

Masamichi Kamihira

Article

Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

Insert

tetracycline-responsive transactivator fused with oxygen-dependent degradation domain

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
WFS1 P724L-Flag-pcDNA3

Plasmid

#13012

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed P 724 to L

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only
pMVS-V1

Plasmid

#183094

Purpose

Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

Depositor

Lars Angenent, Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Inserts

pME2001
thermostable neomycin phosphotransferase gene

Use

Bacterial propagation and conjugative

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET16b-Antp

Plasmid

#86866

Purpose

Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

Depositor

Michael Chin

Article

Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

Insert

Linker-Antp

Tags

Antp

Expression

Bacterial

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFAC-riboB

Plasmid

#168975

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-RABIF-FLAG

Plasmid

#135558

Purpose

Express human RABIF in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pMaCTag-P25

Plasmid

#120036

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9101 - 9150 of 23118 results

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We narrowed to 23,118 results for: Sis