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169,659 results

Showing: 9151 - 9200 of 169659 results

  2. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  5. PylRS-AS

    Plasmid
    #137908
    Purpose
    For incorporation of fluorophenylalanine derivatives
    Depositor
    Wenshe Liu
    Article
    Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    Containing N346A, C348S mutations
    Promoter
    pBAD
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    137908-plasmid-map-sequence-id-272329

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  7. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    human PGK
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

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  11. KAT2A

    Plasmid
    #38914
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KAT2A (KAT2A Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pNBU2_repA2

    Plasmid
    #211911
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211911-plasmid-map-sequence-id-421618

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  19. pFH49

    Plasmid
    #128211
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128211-plasmid-map-sequence-id-252903

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  28. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130635-plasmid-map-sequence-id-255022

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  29. TFORF2303

    Plasmid
    #142019
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CIITA (CIITA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142019-plasmid-map-sequence-id-395917

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  32. ePE6d

    Plasmid
    #229029
    Purpose
    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice
    Depositor
    Li Juan
    Article
    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10
    Insert
    Cas9
    Use
    CRISPR
    Available Since
    Feb. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    229029-plasmid-map-sequence-id-462729

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  34. pENTR-AtTAS1c-D2-B/c

    Plasmid
    #137883
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Use
    Gateway-compatible entry clone
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137883-plasmid-map-sequence-id-275808

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  37. p667-UBC-CDK12-V5-TurboID_IDG-K

    Plasmid
    #135244
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-TurboID (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135244-plasmid-map-sequence-id-311575

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  39. pKB237

    Plasmid
    #170030
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL19 (CCL19 Escherichia coli, Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    170030-plasmid-map-sequence-id-335295

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  45. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Promoter
    RPBSA and TRE promoter
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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  49. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102560-plasmid-map-sequence-id-198595

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Showing: 9151 - 9200 of 169659 results