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pSUPER.retro.puro-shNC

Plasmid

#113535

Purpose

Control retroviral vector.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shNC (scrambled control shRNA)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

IRES-EGFP

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag Raptor

Plasmid

#26633

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3 GATA Luc

Plasmid

#85695

Purpose

Luciferase reporter containing three repeats of the GATA consensus site

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

3xGATA and minimal MT promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

3xGATA and minimal MT promoter

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
ABE8e-NG

Plasmid

#226856

Purpose

Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ABE8e-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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HA-EGFP-HaloTag2

Plasmid

#41742

Depositor

Craig Crews

Article

Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

Insert

Halotag 2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR1A no ccDB (w48-1)

Plasmid

#17398

Purpose

Gateway entry 1A vector, no ccDB gene

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV_Cas9_puro

Plasmid

#65655

Purpose

Retroviral introduction of Cas9 into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Insert

Cas9

Use

CRISPR and Retroviral

Tags

5 ' FLAG tag

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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Pax2

Plasmid

#35002

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Paired box protein Pax-2 (Pax2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pHSV-TK

Plasmid

#165801

Purpose

Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

HSV-TK

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-SpG

Plasmid

#211549

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

Insert

PEmax-SpG

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_GFP

Plasmid

#127108

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

GFP

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-enAsCas12f

Plasmid

#204637

Purpose

Expression of enAsCas12f in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

enAsCas12f

Tags

NLS

Expression

Mammalian

Mutation

D196K, N199K, G276R, N328G, D364R

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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psd44-GqQL

Plasmid

#46826

Purpose

2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

Depositor

Agnese Mariotti

Insert

g protein alpha q (GNAQ Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Q209L (The mutation reduces GTPase activity resul…

Promoter

PGK promoter

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-BE2

Plasmid

#73020

Purpose

Expresses BE2 in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE2

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-TPX2

Plasmid

#31227

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV3_PrME

Plasmid

#207265

Purpose

Mammalian expression of Dengue virus 3 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV3_PrME (POLY Dengue virus 3)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV1_PrME

Plasmid

#207263

Purpose

Mammalian expression of Dengue virus 1 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV1_PrME (POLY Dengue virus 1)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV4_PrME

Plasmid

#207266

Purpose

Mammalian expression of Dengue virus 4 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV4_PrME (POLY Dengue virus 4)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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WT WWP1

Plasmid

#200085

Purpose

WWP1 in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

WT WWP1 (WWP1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-deGFP-8xHis-T500

Plasmid

#178422

Purpose

Expression of deGFP via a T7 Promoter in TXTL

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Insert

deGFP

Use

Synthetic Biology

Tags

8xHis Tag

Expression

Bacterial

Promoter

T7Max

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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eMagAF-EGFP-PM

Plasmid

#162247

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP(C1)-PP1alpha

Plasmid

#44224

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1A (PPP1CA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-neo-hCG

Plasmid

#16574

Depositor

Bert Vogelstein

Article

Shih et al Nat Med. 2000 Jun . 6(6):711-4.

Insert

Beta-hCG (CGB3 Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
mNeon-Vamp2

Plasmid

#186619

Purpose

Encodes Vamp 2 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Vesicle Associated Membrane Protein 2 (VAMP2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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evoBE4-NG

Plasmid

#226852

Purpose

Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

evoBE4max-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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