We narrowed to 7,724 results for: 104
-
-
KG#84
Plasmid#110878PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neuronsDepositorInsertsunc-17beta promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#81
Plasmid#110876PurposeExpresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#83
Plasmid#110877PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
SCYL2 gRNA (BRDN0001145947)
Plasmid#76050Purpose3rd generation lentiviral gRNA plasmid targeting human SCYL2DepositorInsertSCYL2 (SCYL2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SCYL2 gRNA (BRDN0001148262)
Plasmid#76051Purpose3rd generation lentiviral gRNA plasmid targeting human SCYL2DepositorInsertSCYL2 (SCYL2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SCYL2 gRNA (BRDN0001146013)
Plasmid#76052Purpose3rd generation lentiviral gRNA plasmid targeting human SCYL2DepositorInsertSCYL2 (SCYL2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK26 gRNA (BRDN0001149000)
Plasmid#75998Purpose3rd generation lentiviral gRNA plasmid targeting human STK26DepositorInsertSTK26 (STK26 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK26 gRNA (BRDN0001146165)
Plasmid#75999Purpose3rd generation lentiviral gRNA plasmid targeting human STK26DepositorInsertSTK26 (STK26 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK26 gRNA (BRDN0001149381)
Plasmid#76000Purpose3rd generation lentiviral gRNA plasmid targeting human STK26DepositorInsertSTK26 (STK26 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK26 gRNA (BRDN0001148628)
Plasmid#76001Purpose3rd generation lentiviral gRNA plasmid targeting human STK26DepositorInsertSTK26 (STK26 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(L)-Fc-His
Plasmid#72139PurposeExpresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3b (Sema3b Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(S)-Fc-His
Plasmid#72140PurposeExpresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3b (Sema3b Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(S)-AP-His
Plasmid#72014PurposeExpresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3b (Sema3b Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H04
Plasmid#70971PurposeGateway entry cloneDepositorInsertTaf6 (Taf6 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-ZKSCAN1 548-847
Plasmid#69885PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in DrosophilaDepositorUseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
Plasmid#65846PurposeMammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationR5A-K6APromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
Plasmid#65847PurposeMammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationR5D-K6DPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
MOM603
Plasmid#133242Purposeexpresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cellsDepositorInsertkif1a (KIF1A Human)
UseTagsStrepII tag and mScarletExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-BACE-HA
Plasmid#232353PurposeBeta-Secretase to be expressed (1:1) with either ATLAS protein.DepositorInsertBACE (BACE1 Human)
UseAAVTagsHAExpressionMutationPromoterhSynAvailable sinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH N90-beta-catenin
Plasmid#86499PurposeMammalian expression of Myc tagged N90 beta-cateninDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsMycExpressionMammalianMutationDeletion of amino acids 1-90; constitutively acti…PromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Cep27
Plasmid#136809PurposeMammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep27 (HAUS2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-T.1-ABE8e
Plasmid#185668PurposeExpresses eNme2-T.1-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-T.2
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…PromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-HA-NEK7
Plasmid#75142PurposeExpresses N-terminal HA-tagged NEK7 in mammalian cellsDepositorInsertNIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT
Plasmid#163991PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 CARD (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-T.2-ABE8e
Plasmid#185669PurposeExpresses eNme2-T.2-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-T.2
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1244
Plasmid#143777PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPHF20 (PHF20 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-cKit
Plasmid#114293PurposeTET-inducible expression of c-KitDepositorInsertcKit (Kit Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-BICDR1
Plasmid#111858PurposeN-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationR250Q, Optimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro
Plasmid#116876Purposestable TCR expression in human T cellsDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 (CD)
Plasmid#89736PurposeExpresses the CD-domain truncation to mouse TET2 in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-T790M-dual-epegRNA
Plasmid#214100PurposeLentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.DepositorInsertEGFR L858R epegRNA/EGFR T790M epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo_BACE1
Plasmid#227105PurposeBACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assaysDepositorInsertBACE1 (BACE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-MCM7
Plasmid#31212DepositorInsertMCM7 (MCM7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSG5-hEKLF
Plasmid#67835PurposeExpression of human EKLF driven by SV40 promoter for use in activation studiesDepositorInsertEKLF (KLF1 Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCPP5912
Plasmid#128703PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcE-avrE1
UseTagsExpressionBacterialMutationThree synonymous mutations Ala 362 (GCT-GCC), Ala…PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gfp-rt-vasa
Plasmid#167322PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.DepositorInsertrt vasa 3'UTRs (ddx4 rainbow trout)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-PLproCS-MBP
Plasmid#169195PurposeTo express a PLpro substrate (GST-PLproCS-MBP) in E. coliDepositorInsertGST-PLproCS-MBP
UseTagsGST-MBP fusion (PLpro-cleavable) and His6 (intern…ExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only
