We narrowed to 9,400 results for: Coli

1. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234454

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

   Plasmid

   #169191

   Purpose

   To express nsp10-nsp16 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

   Insert

   14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLXI_TRC403 LacZ

   Plasmid

   #111184

   Purpose

   Control inducible vector with LacZ (V5 tagged)

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   lacZ
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLXI_TRC401 LacZ

   Plasmid

   #111183

   Purpose

   Control inducible vector with LacZ (V5 tagged)

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   lacZ
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

   Plasmid

   #209083

   Purpose

   SNCA (Avi tagged) expression along with BirA ligase

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   SNCA (SNCA Human)
   BirA
   

   Use

   Tags

   Avi

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

   Plasmid

   #209084

   Purpose

   Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   NGN2 (Neurog2 Mouse)
   BirA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   hSyn

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_BRD003 Luciferase

   Plasmid

   #117072

   Purpose

   single guide RNA targeting Luciferase

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   Luciferase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HemH-R212A_pET-14b

   Plasmid

   #233262

   Purpose

   Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   HemH (hemH )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 21…

   Promoter

   T7 promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-T20S

   Plasmid

   #110805

   Purpose

   expresses Thermoplasma acidophilium 20S proteasome in E. Coli

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Inserts

   PsmB
   PsmA
   

   Use

   Tags

   tev-His

   Expression

   Bacterial

   Mutation

   N2D and Q2E

   Promoter

   T7 and T7 promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermEp* promoter from S. erythraea

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIMAY

    Plasmid

    #68939

    Purpose

    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

    Depositor

    Tim Foster

    Article

    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

    Type

    Empty backbone

    Use

    Staphylococcal allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL99

    Plasmid

    #78577

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    inducible PnitA-NitR system

    Available Since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Use

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet1_CylLL_CylM

    Plasmid

    #208759

    Purpose

    Expresses modified cytolysin L (mCylLL) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLL
    CylLM
    

    Use

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pALACE

    Plasmid

    #31853

    Depositor

    Yossef Av-Gay

    Article

    Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

    Type

    Empty backbone

    Use

    Escherichia coli-mycobacterium shuttle plasmid

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Acetamidase operone

    Available Since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pRSF-KpAGO207-1251

    Plasmid

    #117854

    Purpose

    Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

    Depositor

    Dinshaw Patel, Kotaro Nakanishi

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Argonaute
    

    Use

    Tags

    6xHis-SUMO encoded in vector

    Expression

    Bacterial

    Mutation

    missing first 206 amino acid residues

    Promoter

    T7

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET303_sumocodo_GFP_codoopti

    Plasmid

    #210393

    Purpose

    Express SUMO-GFP in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Tags

    SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Stephen Quake, Paul Blainey

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSG15-pBAD-tetO-deGFP

    Plasmid

    #102450

    Purpose

    Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD-tetO

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET N6his GFP1-9

    Plasmid

    #182240

    Purpose

    Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Insert

    GFP1-9
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pML433

    Plasmid

    #177190

    Purpose

    Expresses recombinant HyPro enzyme in E. coli

    Depositor

    Eugene Makeyev

    Article

    Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

    Insert

    HyPro enzyme
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJBM4

    Plasmid

    #225291

    Purpose

    E. coli-Campylobacter shuttle vector; TetR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Tet(O) and its putative promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIII/MS2-1

    Plasmid

    #220629

    Purpose

    Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSK+KanaRpsL

    Plasmid

    #20871

    Depositor

    Jiyue Zhu

    Article

    Wang et al Mol Biotechnol. 2009 May;42(1):110-6. do

    Insert

    KanamycinResistance-RpsL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSAP

    Plasmid

    #206429

    Purpose

    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZA16mflon

    Plasmid

    #75439

    Purpose

    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    mf-lon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-6xHis-mCherry(-6)

    Plasmid

    #205045

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    mCherry(-6)
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a human Tankyrase2 full length Y920E

    Plasmid

    #132639

    Purpose

    expresses human Tankyrase2 full length Y920E in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

    Insert

    Tankyrase2 Y920E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge