We narrowed to 54,258 results for: Ran

1. plc242-GFP-Nprl2

   Plasmid

   #87051

   Purpose

   mammalian expression of Nprl2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   Nprl2 (NPRL2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p CIneo-RL-Let7-perf

   Plasmid

   #115367

   Purpose

   Expression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTR

   Depositor

   Witold Filipowicz, Ramesh Pillai

   Article

   Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCasTns(Vch)-entry(BsaI)

   Plasmid

   #190273

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no

   Promoter

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AY42_pS.Donor.R5

   Plasmid

   #100291

   Purpose

   CCR5-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdv/TERT

   Plasmid

   #26744

   Depositor

   Bingliang Fang

   Article

   Gu et al Cancer Res. 2000 Oct 1. 60(19):5359-64.

   Insert

   human TERT promoter (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPRIME-dsRed-shvgat

   Plasmid

   #71385

   Purpose

   Expresses dsRed and shRNA against vgat under the control of the CMV promoter in eukaryotic cells

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   CMV promoter-dsRed-mir30-shvgat
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer/promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRuby2-MyosinIIA-C-18

   Plasmid

   #55906

   Purpose

   Localization: Cytoskeleton, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   MyosinIIA
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pColE1_sgRNA_M1S_AmpR

   Plasmid

   #119153

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pColE1_sgRNA_M1NS_AmpR

   Plasmid

   #119155

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTetQCas-BsaI

    Plasmid

    #170629

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGG-PM-TurboID

    Plasmid

    #209395

    Purpose

    Transiently expressing TurboID fused with a plasma menbrane targeting peptide in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW2-Smed-Slit-1

    Plasmid

    #182264

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

    Depositor

    Daniel Lobo

    Article

    Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

    Insert

    Smed-Slit-1
    

    Use

    Gibson cloning vector for synthesis of single and…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK527a

    Plasmid

    #110606

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pDrop

    Plasmid

    #184872

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLV GG hUbV-EGFP

    Plasmid

    #216161

    Purpose

    Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAWH

    Plasmid

    #133894

    Purpose

    Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-CamK2-tTA

    Plasmid

    #210731

    Purpose

    AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

    Depositor

    Wei Xu

    Article

    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    tetracycline-controlled transactivator (tTA)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CamK2

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRhaQCas-BsaI

    Plasmid

    #170631

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

    Plasmid

    #191675

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6X his and flag

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. rTetR-GFP-VPR

    Plasmid

    #183918

    Purpose

    VPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    rTetR-GFP-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RP2-EGFP

    Plasmid

    #86073

    Purpose

    A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PX459-APP-KO

    Plasmid

    #176487

    Purpose

    To knockout APP gene

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Dapp1-PH pET11a

    Plasmid

    #71376

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Dapp1-PH (DAPP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 148-273

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Clover-Rab5a-7

    Plasmid

    #56530

    Purpose

    Localization: Ras GTPase, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Rab5a
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pColE1_sgRNA_M4NS_AmpR

    Plasmid

    #119156

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GST-RP2

    Plasmid

    #86072

    Purpose

    A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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