We narrowed to 12,055 results for: cel.2

1. PCBP4_3xKH_pGEX

   Plasmid

   #135156

   Purpose

   Recombinant expression of PCBP4 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PCBP4 (PCBP4 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. JARID2-KO-gRNA-1

   Plasmid

   #131328

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

3. EED-KO-gRNA-1

   Plasmid

   #131321

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

4. RBMS2_2xRRM_pGEX

   Plasmid

   #135132

   Purpose

   Recombinant expression of RBMS2 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBMS2 (RBMS2 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-NT-3/D15A

   Plasmid

   #73035

   Purpose

   Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Insert

   NT-3/D15A (NTF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Aspartic acid in position 15 of the matur…

   Promoter

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV sMTase (eGFP)

   Plasmid

   #89930

   Purpose

   Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC-IRES-MN
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   deactivated Cas9

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKrox24(2xD-E)DsRed

   Plasmid

   #200111

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. tetO.Nfia.Blast

   Plasmid

   #117270

   Purpose

   Expresses Nfia and blasticidin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfia (Nfia Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lifeact-7-iRFP670

   Plasmid

   #103032

   Purpose

   Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LifeAct-14 (LA-14)

    Plasmid

    #158750

    Purpose

    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    LifeAct-14
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Deletion mutation for last three amino acids from…

    Promoter

    CMV immediate early

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-GFP-WT1 +KTS N terminal HA

    Plasmid

    #217639

    Purpose

    Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 +KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-GFP-WT1 -KTS N terminal HA

    Plasmid

    #217641

    Purpose

    Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    p.408_410delKTS

    Promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-OC-IRES-BSD

    Plasmid

    #53118

    Purpose

    To create stable cell clones with high-level expression of Cas9 and OCT1

    Depositor

    Wensheng Wei

    Article

    Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Inserts

    OCT1 (POU2F1 Human)
    Cas9
    

    Use

    Lentiviral

    Tags

    IRES-BSD, NLS, and P2A

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMXS-IRES-Blast coSHMT2

    Plasmid

    #106299

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-mPCSK8

    Plasmid

    #122677

    Purpose

    Expresses mouse PCSK8 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB-RTP2-Integration

    Plasmid

    #129459

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP2 (RTP2 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-HCF-1rep1E10A

    Plasmid

    #84607

    Purpose

    Plasmid contains HCF-1rep1E10A with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1E10A (HCFC1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-HCF-1rep1wt

    Plasmid

    #84606

    Purpose

    Plasmid contains HCF-1rep1wt with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1wt (HCFC1 Human)
    

    Use

    Tags

    GST-TEV and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hs-lambda btl

    Plasmid

    #17581

    Depositor

    Denise Montell

    Article

    Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

    Insert

    lambda-btl (btl Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)

    Plasmid

    #61943

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Residues 2-39 deleted resulting in construct star…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pENTR-GFP-WT1 +KTS C terminal HA

    Plasmid

    #217640

    Purpose

    Entry vector with WT1 +KTS mutant with C terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 +KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR-GFP-WT1 -KTS C terminal HA

    Plasmid

    #217642

    Purpose

    Entry vector with WT1 -KTS mutant with C terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    p.408_410delKTS

    Promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKrox24(2xD-E)dTomato

    Plasmid

    #200110

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST-hMeCP2-GFP

    Plasmid

    #48078

    Purpose

    Mammalian expression of GFP tagged MeCP2-WT

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FGFR1-optoDroplet

    Plasmid

    #111509

    Purpose

    For optogenetic clustering of FGFR1

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2, FUS, Fusion Red, and Myristoylation sequenc…

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ss-SNAP-TMD-NS3-Gal4

    Plasmid

    #112279

    Purpose

    Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    SNAP-TMD-NS3-Gal4
    

    Use

    Tags

    AU1 (N term on NS3) and HA (C term on NS3)

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTXB1-hMeCP2

    Plasmid

    #48091

    Purpose

    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mxe intein/chitin binding domain tagged

    Promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

29. PDPN-bio-His

    Plasmid

    #52313

    Purpose

    Expresses full-length Podoplanin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PDPN (PDPN Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDEST-hMeCP2-GFP-R270X

    Plasmid

    #48079

    Purpose

    Mammalian expression of GFP tagged MeCP2-R270X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    expresses AT-Hook R270X

    Promoter

    CMV

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid

    #140228

    Purpose

    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    RRM2 endogenous promoter

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs2-RAC1

    Plasmid

    #86138

    Purpose

    Retrolviral vector expressing RAC1-IRES-Bsd-p2A-RFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

33. VIPR1

    Plasmid

    #51865

    Purpose

    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    VIPR1 (VIPR1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HOXA9-MSCV-CD24

    Plasmid

    #20975

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

    Inserts

    HOXA9 (Hoxa9 Mouse)
    CD24 (CD24 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Full length cDNA with ~1.1kb 3'UTR with 2 pu…

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR4 PARP2 E614Q

    Plasmid

    #192545

    Purpose

    pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    changed Glu 614 to Gln

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMXS-IRES-Blast SHMT2res

    Plasmid

    #106301

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations destroy sgRNA targeting sites

    Promoter

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDEST-hMeCP2-GFP-G273X

    Plasmid

    #48080

    Purpose

    Mammalian expression of GFP tagged MeCP2-G273X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    expresses AT-Hook G273X

    Promoter

    CMV

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMX-Gal4-hMeCP2-R270X

    Plasmid

    #48083

    Purpose

    Mammalian expression of Gal4 tagged MeCP2-R270X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    expresses AT-Hook R270X

    Promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid

    #140227

    Purpose

    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HRE (Hypoxia inducible)

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMX-Gal4-hMeCP2-R111G

    Plasmid

    #48085

    Purpose

    Mammalian expression of Gal4 tagged MeCP2-WT-R111G

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    R111G mutant MeCP2

    Promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

    Plasmid

    #124871

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMX-Gal4-hMeCP2-G273X

    Plasmid

    #48084

    Purpose

    Mammalian expression of Gal4 tagged MeCP2-G273X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    expresses AT-Hook G273X

    Promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

    Plasmid

    #145144

    Purpose

    Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

    Depositor

    Johanna Ivaska

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pENTR-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233157

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMXs-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233163

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAc5.1B-EGFP-DmMe31B-Q294AN296F-dsRNAres_W

    Plasmid

    #147840

    Purpose

    Insect Expression of DmMe31B-Q294AAN296F-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmMe31B-Q294AAN296F-dsRNAres (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAc5.1B-EGFP-DmMe31B-Q294AN296FR347A-dsRNAres_W

    Plasmid

    #147841

    Purpose

    Insect Expression of DmMe31B-Q294AAN296FR347A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmMe31B-Q294AAN296FR347A-dsRNAres (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAc5.1B-EGFP-DmMe31B-R347A-dsRNAres_W

    Plasmid

    #147842

    Purpose

    Insect Expression of DmMe31B-R347A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmMe31B-R347A-dsRNAres (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAc5.1B-EGFP-DmMe31B-S291A-dsRNAres_W

    Plasmid

    #147843

    Purpose

    Insect Expression of DmMe31B-S291A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmMe31B-S291A-dsRNAres (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pPlacPbuCas13b-gRNA-NT

    Plasmid

    #184838

    Purpose

    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge