We narrowed to 13,226 results for: 109

1. IDP428

   Plasmid

   #87014

   Purpose

   genomic Sindbis virus plasmid for use in SYNseq PLA screen

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Inserts

   CLIP-Nrx1B-1xλN(c)
   mCherry
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BC-A1-016

   Plasmid

   #78704

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BC-A1-015

   Plasmid

   #78703

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BC-A1-008

   Plasmid

   #78695

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BC-A1-007

   Plasmid

   #78694

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. const #7-RFP

   Plasmid

   #81081

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Paul Adams, Jay Keasling

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23109

   Available since

   Aug. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBW215J105-Amp32C

   Plasmid

   #78650

   Purpose

   pSB3K3 carrying J105-30hrpR-32hrpS-t-hrpL-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   J105-30hrpR-32hrpS-t-hrpL-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pBW212J114-Amp32C

   Plasmid

   #78647

   Purpose

   pSB3K3 carrying J114-30hrpR-32hrpS-t-hrpL-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   J114-30hrpR-32hrpS-t-hrpL-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

9. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycLAM

   Plasmid

   #71807

   Purpose

   Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-LAM
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC

    Plasmid

    #71808

    Purpose

    Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-PC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTd-4-15-13-1

    Plasmid

    #63826

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTd-4-15-13-2

    Plasmid

    #63827

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pTd-4-15-13-3

    Plasmid

    #63828

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pTd-4-15-13-4

    Plasmid

    #63829

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pTd-4-15-13-5

    Plasmid

    #63830

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pTd-4-15-13-6

    Plasmid

    #63831

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pTd-4-15-13-7

    Plasmid

    #63832

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pTd-4-15-13-8

    Plasmid

    #63833

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pTd-4-15-13-9

    Plasmid

    #63834

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTd-4-15-13-11

    Plasmid

    #63836

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pSCKtheoRaj12

    Plasmid

    #69942

    Purpose

    pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR

    Depositor

    Alfonso Jaramillo

    Article

    Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    TheoHHAzRaj12,cisRaj12,sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSCKtheoRaj11

    Plasmid

    #69939

    Purpose

    pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR

    Depositor

    Alfonso Jaramillo

    Article

    Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    TheoHHAzRaj11,cisRaj11,sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSCKbreak12

    Plasmid

    #69938

    Purpose

    pSTC2,cisRaj12-sfGFP, KanR

    Depositor

    Alfonso Jaramillo

    Article

    Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    CisRaj12-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFOSGE_COF1G12

    Plasmid

    #70860

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    mrn (mrn Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pFOSGE_COF1E01

    Plasmid

    #70777

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    RpLP2 (RpLP2 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pGL3-PIM2mut2

    Plasmid

    #51850

    Purpose

    promoter region of PIM2 lacking the forkhead binding site #1

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    PIM2 (PIM2 Human)
    

    Use

    Luciferase; Reporter vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

27. psicheck-2-Phb

    Plasmid

    #48162

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Phb (Phb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

28. psicheck-2-Slc25a15 mod

    Plasmid

    #48161

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Slc25a15
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

29. UAS-Myc-dASUN

    Plasmid

    #47036

    Purpose

    Drosophila expression of tagged dASUN

    Depositor

    Laura Lee

    Article

    Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

    Insert

    Asunder (asun Fly)
    

    Use

    Tags

    Myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

30. psicheck-2-Mrpl1

    Plasmid

    #48166

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of MRPL11 (Mrpl11 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

31. psicheck-2-Slc25a15

    Plasmid

    #48160

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Slc25a15
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

32. CS2-HA-dASUN

    Plasmid

    #47041

    Purpose

    Expression of tagged-dASUN

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

    Insert

    Asunder (asun Fly)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

33. TV3-mCHY-dASUN

    Plasmid

    #47034

    Purpose

    Drosophila testes specific expression of fluorescent dASUN

    Depositor

    Laura Lee

    Article

    Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

    Insert

    Asunder (asun Fly)
    

    Use

    Tags

    mCHY

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

34. TV3-eGFP-dASUN

    Plasmid

    #47033

    Purpose

    Drosophila testes specific expression of fluorescent dASUN

    Depositor

    Laura Lee

    Article

    Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

    Insert

    Asunder (asun Fly)
    

    Use

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pMB38-HF380deltaMTBD

    Plasmid

    #37333

    Depositor

    Takahide Kon

    Article

    Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

    Insert

    Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
    

    Use

    Dictyostelium expression

    Tags

    His6 and FLAG tag

    Expression

    Mutation

    deleted amino acids 1-1387 and replaced amino aci…

    Promoter

    TRE-Pmin

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pIS57 PGM2 3'UTR

    Plasmid

    #14491

    Depositor

    David Bartel

    Article

    Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

    Insert

    PGM2 3'UTR (PGM2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK430

    Plasmid

    #155377

    Purpose

    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMSCV-dCasRx-METTL3-PURO

    Plasmid

    #174218

    Purpose

    Expresses dCasRx-METTL3 in mammalian cell

    Depositor

    Qi Xie

    Article

    Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

    Insert

    dCasRx-METTL3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Cyto-roGFP

    Plasmid

    #49435

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    roGFP2
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a-MSP1D1delltaH5

    Plasmid

    #71714

    Purpose

    Membrane scaffold protein (MSP), helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Lenti-CMV-PRB

    Plasmid

    #206165

    Purpose

    pMVP Gateway destination vector (3rd generation lentiviral vector) containing human full length PRB gene

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    Progesteron receptor B (PGR Human)
    

    Use

    Lentiviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. KP501: pMVP (L5-L4) eYFP-P2A

    Plasmid

    #121714

    Purpose

    pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eYFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDule-tfmF A65V S158A

    Plasmid

    #85484

    Purpose

    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32V F108W Q109M I159M with 65Val and 158Ala

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. ires-eGFP(+HAs) donor-1

    Plasmid

    #87865

    Purpose

    Express eGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRRL-mU6-GFPGO2-IRES-mScarletI

    Plasmid

    #136896

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO2-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    2xNLS on GFP, 3xNLS on mScarlet

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pKCcas9dO

    Plasmid

    #62552

    Purpose

    High efficiency Streptomyces genome editing by CRISPR/Cas9 system

    Depositor

    Weihong Jiang

    Article

    Huang et al Acta Biochim Biophys Sin (Shanghai). 201

    Inserts

    Scocas9
    sgRNA ()
    act-orf4 homology arm
    

    Use

    CRISPR; Bacterial genome editing

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    j23119 and ptipA

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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