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Showing: 9401 - 9450 of 32215 results

  11. pAL3

    Plasmid
    #114673
    Purpose
    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
    Depositor
    William Studier
    Article
    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6
    Type
    Empty backbone
    Use
    Tags
    optional Ser-Gly-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    114673-plasmid-map-sequence-id-254326

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  18. pZH520

    Plasmid
    #102673
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102673-plasmid-map-sequence-id-199588

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  19. SHC003BSD

    Plasmid
    #133300
    Purpose
    The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.
    Depositor
    Jingshi Shen
    Article
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133300-plasmid-map-sequence-id-261777

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  23. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  34. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  36. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162457-plasmid-map-sequence-id-321472

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Showing: 9401 - 9450 of 32215 results