We narrowed to 126,872 results for: CIL

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pLEX_305-N-dTAG

Plasmid

#91797

Purpose

Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Publication

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pC015 - dLwCas13a-NF

Plasmid

#91905

Purpose

Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

Expression

Mammalian

Mutation

R474A and R1046A

Promoter

CAG

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEP4 E02S CK2M EN2L

Plasmid

#20924

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

Depositor

James Thomson

Publication

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)

Use

Episomal/ebna

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCSCMV:tdTomato

Plasmid

#30530

Purpose

mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.

Depositor

Gerhart Ryffel

Publication

Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.

Insert

tdTomato

Expression

Mammalian

Mutation

R415H

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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pCDNA3-Myc3-Nrf2

Plasmid

#21555

Purpose

Mammalian expression of human Nrf2 with Myc tag

Depositor

Yue Xiong

Publication

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

myc3

Expression

Mammalian

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pLEX_305-C-dTAG

Plasmid

#91798

Purpose

Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Publication

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pHR_PGK_antiCD19_synNotch_Gal4VP64

Plasmid

#79125

Purpose

Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Publication

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

antiCD19_synNotch_Gal4VP64

Use

Lentiviral

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

Plasmid

#20298

Purpose

Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Has service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

channelrhodopsin-2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

humanized ChR2 gene with histidine 134 changed to…

Promoter

EF1alpha

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

363 Articles

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pEF.myc.ER-E2-Crimson

Plasmid

#38770

Purpose

In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

Depositor

Benjamin Glick

Publication

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Tags

ER retention signal (KDEL), ER signal sequence (M…

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Publication

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Publication

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

89 Articles

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pCAX APP Swe/Ind

Plasmid

#30145

Depositor

Dennis Selkoe, Tracy Young-Pearse

Publication

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

human APP 695 Swedish/Indiana mutation (APP Human)

Expression

Mammalian

Mutation

Swedish and Indiana; K595N, M596L and V642F

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pTriEx-HTNC

Plasmid

#13763

Purpose

For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

Depositor

Klaus Rajewsky

Publication

Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

Insert

His-TAT-NLS-Cre

Use

Baculovirus

Tags

His Tag, NLS, and TAT

Expression

Bacterial

Promoter

p10 promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCR4-24XMS2SL-stable

Plasmid

#31865

Depositor

Robert Singer

Publication

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

24xMS2 Stem Loops - Stable

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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Cre-IRES-PuroR

Plasmid

#30205

Purpose

Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

Depositor

Darrell Kotton

Publication

Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

Insert

Cre recombinase (cre Enterobacteria phage P1)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pC0046-EF1a-PspCas13b-NES-HIV

Plasmid

#103862

Purpose

Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PspCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Available since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

26 Articles

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