We narrowed to 17,013 results for: Por

1. TRE-UBC-mCD55-T2A-GFP

   Plasmid

   #205450

   Purpose

   lentiviral plasmid for expression of mouse CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD55 (Cd55 Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/MPL-V5

   Plasmid

   #204531

   Purpose

   Mammalian expression of human MPL-V5

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   MPL-V5 (MPL Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

   Plasmid

   #224490

   Purpose

   Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Golgi-mTagBFP-P2A-H2A-iRFP
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLIK 3xFLAG-KPNA2 zeo

   Plasmid

   #192330

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-KPNA2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KPNA2 (KPNA2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CD8-mCherry-FRB

   Plasmid

   #100738

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

   Plasmid

   #232244

   Purpose

   Mammalian expression of N581K RLIM-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   N581K RLIM-GFP-IRES-mCherry (RLIM Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   N581K

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

   Plasmid

   #232240

   Purpose

   Mammalian expression of REX1[degron]-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   REX1 (aa 70-85)

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast CHKA

   Plasmid

   #218540

   Purpose

   human CHKA cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   CHKA (CHKA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRX-IP/SECFP-hATG16A1

   Plasmid

   #58994

   Purpose

   Expresses SECFP-hATG16A1 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
   

   Use

   Retroviral

   Tags

   SECFP

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZ403

    Plasmid

    #208113

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-PuroTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-PuroTK
    

    Tags

    P2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PM-RspA-CFAST

    Plasmid

    #233601

    Purpose

    Expression of RspA-CFAST on the PM facing the cytosol

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-CFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

    Plasmid

    #194686

    Purpose

    CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-epsin(144-575)-GFP

    Plasmid

    #100733

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    epn1 (Epn1 Mouse)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    clathrin binding middle part aa 144-575

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215326

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    dNTP63 Promoter
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    dNTP63

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK 3xFLAG-KPNB1 zeo

    Plasmid

    #192326

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-KPNB1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNB1 (KPNB1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHD-SPARC2-S-LexA::p65

    Plasmid

    #133564

    Purpose

    Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Mutation

    codon optimized for human

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. LUC01 (L/Sec/P)

    Plasmid

    #112144

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U)

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCW57.1 tTA UQCRC2

    Plasmid

    #177978

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Silent point mutations to UQCRC2 sgRNA target

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAd-CCN1

    Plasmid

    #12519

    Depositor

    Tong-Chuan He

    Article

    Si et al Mol Cell Biol. 2006 Apr . 26(8):2955-64.

    Insert

    CCN1 (Ccn1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pAAV-hSyn-GRAB_OTmut

    Plasmid

    #185388

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWZ854

    Plasmid

    #208116

    Purpose

    mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mNeonGreen-HPRT1-Neo
    

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWZ852

    Plasmid

    #208114

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-HygTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-HygTK
    

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-Y1175F-VEGFR2-mCherry

    Plasmid

    #139608

    Purpose

    Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. nuc-GafD-mTurboID-HA

    Plasmid

    #184641

    Purpose

    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    nuc-GlycoID-HA
    

    Tags

    3xHA tag and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    GafD -> expressed 22-178 ; BirA* -> express…

    Promoter

    CMV

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSynapsin- soCoChR-GFP

    Plasmid

    #107708

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Has Service

    AAV9

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LAMP1-mCherry-FRB*

    Plasmid

    #207145

    Purpose

    Lysosome-targeted rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Tags

    FRB* (T2098L mutant of FKBP-rapamycin binding dom…

    Expression

    Mammalian

    Mutation

    Silent mutation

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Cox7c-pHLuorin-sems

    Plasmid

    #107766

    Purpose

    labeling of mitochondrial cytochrome c oxidase, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit Cox7c fused to pHluorin (COX7C Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

    Plasmid

    #174620

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

    Depositor

    Volker Busskamp

    Article

    Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

    Insert

    enhanced green fluorescent protein
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHD-SPARC2-D-LexA::p65

    Plasmid

    #133562

    Purpose

    Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

    Plasmid

    #194690

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-RON-V5/HIS

    Plasmid

    #201983

    Purpose

    expression of human RON receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RON receptor tyrosine kinase (MST1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PGK EGFP-PPM1H-WT

    Plasmid

    #200084

    Purpose

    Mammalian expression of wild-type PPM1H

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    PPM1H (PPM1H Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

    Plasmid

    #194691

    Purpose

    hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge