159,157 results

1. AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908)

   Plasmid

   #224052

   Purpose

   AiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-HA-USP49

   Plasmid

   #22586

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP49 (USP49 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

3. phOct4-EGFP

   Plasmid

   #38776

   Depositor

   Wei Cui

   Article

   Gerrard et al Stem Cells. 2005;23(1):124-33.

   Insert

   Oct-4 promoter (POU5F1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   human OCT4

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1844

   Plasmid

   #144313

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RB1 (RB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVHA E2F1

   Plasmid

   #24225

   Purpose

   Mammalian expression of human E2F1 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. c-myc promoter (Del 1)

   Plasmid

   #16601

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 1) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-1 (XmnI->PvuII)

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET-mEosEM

   Plasmid

   #132708

   Purpose

   application in Correlative light-electron microscopy

   Depositor

   Pingyong Xu

   Article

   Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   mEosEM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-PGK-destination

   Plasmid

   #60436

   Purpose

   Destination vector with PGK - Hygromycin selection marker

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HDAC7 Flag

   Plasmid

   #13824

   Purpose

   Mammalian expression of human histone deacetylase 7 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC7 (HDAC7 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid

    #174896

    Purpose

    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NA20 LN16

    Plasmid

    #101870

    Purpose

    Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

    Depositor

    Paul Clapham

    Article

    Peters et al J Virol. 2004 Jul;78(13):6915-26.

    Insert

    Env (env )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

12. h-Menkes

    Plasmid

    #196953

    Purpose

    Plasmid expressing an antigen targeting the Menke's disease copper ATPase

    Depositor

    James Trimmer

    Insert

    Copper ATPase 1 (ATP7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTF102

    Plasmid

    #134774

    Purpose

    A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.

    Depositor

    Kan Wang

    Article

    Frame et al Plant Physiol. 2002 May;129(1):13-22. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC18-mini-Tn7T-LAC

    Plasmid

    #64965

    Purpose

    mini-Tn7 vector with Ptac expression cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGreen2x35SLUC

    Plasmid

    #108228

    Purpose

    Plant expression as translational fusions to FLUC with RLUC normalization

    Depositor

    Alejandro Ferrando

    Article

    Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGG-NFIX1

    Plasmid

    #112702

    Purpose

    Expression of NFIX1 protein and GFP

    Depositor

    Richard Gronostajski

    Insert

    Nuclear Factor I X1 (Nfix Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVUT-tTR-KRAB

    Plasmid

    #11651

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Has Service

    Cloning Grade DNA

    Insert

    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAWP78-PVCpnf1-16

    Plasmid

    #198271

    Purpose

    Unmodified pPVC; contains WT PVC structural/accessory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf1-16
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

    Plasmid

    #80840

    Purpose

    Modified helper phage

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene II
    M13 gene X
    M13 gene V
    M13 gene VII
    M13 gene IX
    M13 gene VIII
    M13 gene I
    M13 gene XI
    M13 gene IV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T7-RelA(S536E)

    Plasmid

    #24156

    Depositor

    Warner Greene

    Article

    Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

    Insert

    RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    S536E

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pCGN N-Ras wt

    Plasmid

    #14723

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    N-ras (NRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-GFP1-10

    Plasmid

    #78591

    Purpose

    Express mCherry-GFP1-10 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    GFP 1-10
    

    Use

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PEmax_2star_pet21A

    Plasmid

    #227675

    Purpose

    Expression plasmid for PEMAX** protein in bacteria.

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    PEmax_2_star
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMP71-BFP

    Plasmid

    #141355

    Purpose

    pMP71 expression vector containing BFP

    Depositor

    Ton Schumacher

    Article

    Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

    Insert

    tagBFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Bxb1-GA Donor_hEF1a

    Plasmid

    #229779

    Purpose

    Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENN.AAV.CamKII0.4.eGFP.WPRE.rBG

    Plasmid

    #105541

    Purpose

    AAV expression of EGFP from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1 and AAV5

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamkII

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEG BacMam C term 3C eGFP StrepII

    Plasmid

    #160686

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    3C eGFP StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. tetO-SOX9

    Plasmid

    #170703

    Purpose

    doxycycline-inducible overexpression of SOX9

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA4TO-24xGCN4_v4-BFP-24xPP7

    Plasmid

    #74929

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-BFP-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1-FRB-LgBiT

    Plasmid

    #182650

    Purpose

    FRB-LgBiT

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    FRB-LgBiT fusion
    

    Use

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFUW-TetO-ZFP36

    Plasmid

    #178438

    Purpose

    doxycycline-inducible expression of Human ZFP36 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    ZFP36 (ZFP36 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. YFP-CD82

    Plasmid

    #1819

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    CD82 (CD82 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-UtrCH

    Plasmid

    #26737

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261).…

    Promoter

    CMV IE94

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pECE-M2-BAIAP2 wt

    Plasmid

    #31656

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 (BAIAP2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PS-CFP2-TOMM20-N-10

    Plasmid

    #57250

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

40. EGFP-BAF

    Plasmid

    #101772

    Purpose

    Expresses EGFP tagged BAF in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge