159,461 results

1. pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

   Plasmid

   #195552

   Purpose

   Expresses Thbs1 under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   short GFAP

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. H1.99.luc

   Plasmid

   #193663

   Purpose

   Expresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99)

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated Pol III H1 promoter (H1.99) + 41 nucleotides
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE

   Plasmid

   #189643

   Purpose

   16S rRNA Right_G1397C_ABE

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

   Insert

   TALE and DddAtox and TadA 8e
   

   Use

   TALEN

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPS5A-PTP-3Xflag-16S rRNA Left_G1397N

   Plasmid

   #189641

   Purpose

   16S rRNA Left_G1397N

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

   Insert

   TALE and DddAtox
   

   Use

   TALEN

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACE-GIRK4-mC-S

   Plasmid

   #172427

   Purpose

   Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Use

   Tags

   mCherry Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-747pUC19-tNGFR-P2A-CD7

   Plasmid

   #186086

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CD7 (CD7 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mt-DdCBE G1333-C

   Plasmid

   #174561

   Purpose

   mitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

   Depositor

   Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

   Insert

   DdCBE G1333-C
   

   Use

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   PcUbi

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mt-DdCBE G1333-N

   Plasmid

   #174560

   Purpose

   mitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

   Depositor

   Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

   Insert

   DdCBE G1333-N
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   PcUbi

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK_TFAP2b_enh_226_Citrine

   Plasmid

   #130618

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   TFAP2b_enh_226
   

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ESAM-bio-His

    Plasmid

    #51751

    Purpose

    Expresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ESAM (ESAM Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30-GBFusion1

    Plasmid

    #133438

    Purpose

    Streptococcus GB1 domain in vector for bacterial expression.

    Depositor

    Gerhard Wagner

    Article

    Zhou et al J Biomol NMR. 2001 May . 20(1):11-4.

    Insert

    GB1 domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    This fragment encodes only aa 1 to 56.

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #179686

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T1413I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-HA-hYTHDC1

    Plasmid

    #85167

    Purpose

    Expression of FLAG-HA-tagged human YTHDC1

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Flag and Ha

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFD5

    Plasmid

    #73914

    Purpose

    multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    dU6-3:tRNA:gRNA:tRNA:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. EMTB-3XGFP

    Plasmid

    #26741

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    3x EGFP and Myc

    Expression

    Mammalian

    Mutation

    Only contains the microtubule binding-domain of e…

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. mRFP-Rab7

    Plasmid

    #14436

    Purpose

    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 7 (RAB7A Canis familialis)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    3 silent mutations near the C-terminus

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ecoli PancACe

    Plasmid

    #215712

    Purpose

    Expresses acetyl-CoA biosensor in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. FASTHDR-Cterm-mClover3-Puromycin

    Plasmid

    #167205

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge