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pB1H2w2-zif268

Plasmid

#18045

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

zif268

Expression

Bacterial

Mutation

Zif268 is fused to the omega subunit of RNA polym…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GLI K12

Plasmid

#16419

Depositor

Bert Vogelstein

Publication

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI (GLI1 Human)

Expression

Bacterial

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pQLinkN

Plasmid

#13670

Depositor

Konrad Buessow

Publication

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Expression

Bacterial

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAdEasy Flag GCN5

Plasmid

#14106

Depositor

Pere Puigserver

Publication

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

E189G and K195N

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-HA-14-3-3 epsilon

Plasmid

#13273

Depositor

Michael Yaffe

Insert

14-3-3 epsilon (YWHAE Human)

Tags

HA

Expression

Mammalian

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
mTLR4

Plasmid

#13085

Depositor

Ruslan Medzhitov

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TLR4 (Tlr4 Mouse)

Expression

Mammalian

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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1013 pcDNA3 T7 Akt1 T308A S473A

Plasmid

#9030

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1 T308A S473A (AKT1 Human)

Expression

Mammalian

Mutation

T308A S473A

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLX_305_Ovalbumin

Plasmid

#184924

Purpose

Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

Depositor

Arlene Sharpe

Publication

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

Ovalbumin (OVAL Chicken)

Use

Lentiviral

Expression

Mammalian

Mutation

Our vector has an A188T mutation that does not im…

Promoter

PGK

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Publication

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDisCoTune

Plasmid

#173713

Purpose

Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Depositor

Morten Norholm

Publication

Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

Inserts

ERV1 (ERV1 Budding Yeast)
PDI

Expression

Bacterial

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Publication

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3-sACE2(WT)-Fc(IgG1)

Plasmid

#145163

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

Plasmid

#140001

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human codon optimized CBE4max SpCas9-NG with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

Promoter

CAG

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

Plasmid

#135391

Purpose

Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

Depositor

Fan Wang

Insert

TeLC-P2A-EYFP

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCMV-15F11-HA-Halo

Plasmid

#129592

Purpose

Encodes anti-HA scFv (anti-HA frankenbody) fused with HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pET_mNG2(1-10)_32aalinker_mNG2(11)

Plasmid

#82611

Purpose

Bacterial expression vector for split mNeonGreen2 screening

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)

Expression

Bacterial

Mutation

split before mutations

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-PIK3CA-H1047R

Plasmid

#116500

Purpose

Lentiviral expression of PIK3CA H1047R

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

H1047R

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Publication

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmGFP-ADAR2

Plasmid

#117929

Purpose

ADAR2 expression plasmid.

Depositor

Kumiko Ui-Tei

Publication

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR2 (ADARB1 Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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