12,329 results

1. [UBC][EIF4e][GG cloning]

   Plasmid

   #97189

   Purpose

   EIF4e plasmid with Pum cloning site

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Insert

   EIF4E variant 1 (EIF4E Human)
   

   Use

   Synthetic Biology

   Tags

   empty Pum cloning site

   Expression

   Mammalian

   Promoter

   UBC (ubiquitin C)

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL705

   Plasmid

   #140375

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-5BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB170

   Plasmid

   #101669

   Purpose

   Opto-T7RNAP*(69), half expr.

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(69), half expr.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSB-hsa-miR-138-5p

   Plasmid

   #103233

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-138-5p target
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbE2k-pTet-gRNAwcaF159

   Plasmid

   #122259

   Purpose

   aTc inducible gRNA expression plasmid

   Depositor

   Chueh Loo Poh

   Article

   Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

   Insert

   gRNAwcaF159
   

   Use

   Synthetic Biology

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbA6a-RFP

   Plasmid

   #35289

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBP-Bba_K822000

   Plasmid

   #74094

   Purpose

   L-Lactate inducible promoter from E. coli MG1655

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Bba_K822000 (L-Lactate promoter - E. coli MG1655)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   NA

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGG426_UV5

   Plasmid

   #165608

   Purpose

   Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   D1135V, S1136R, D1332K and R1333G mutations in Sp…

   Promoter

   lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA0.002

   Plasmid

   #119560

   Purpose

   Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT103

    Plasmid

    #25087

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    PompC-lacZ
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGM9121_GFP11

    Plasmid

    #153508

    Purpose

    GFP11 C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    GFP11
    

    Use

    Synthetic Biology

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAL-pyk_T

    Plasmid

    #74070

    Purpose

    pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pyk (T)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

13. MXS_PGK

    Plasmid

    #62414

    Purpose

    MXS_chaining vector with PGK

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7_circRAJ31

    Plasmid

    #69931

    Purpose

    Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    pT7_circRAJ31
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

    Plasmid

    #160616

    Purpose

    TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXTL-T7max-HisGFPHiBiT

    Plasmid

    #188316

    Purpose

    Expression of GFP-HiBiT via T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    GFP-HiBiT fusion
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB1975 (pUC19-PTEF1-PPGK1)

    Plasmid

    #73296

    Purpose

    Example plasmid containing a bidirectional promoter

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    pTEF1-pPGK1
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZS1[TreR-T]

    Plasmid

    #60754

    Purpose

    Contains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    TreR-T
    GalS-T
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Chimeric LacI/GalR repressor with the TAN DBD and…

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108845

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GAI

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBP-TL4

    Plasmid

    #72988

    Purpose

    RBS_H5

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    H5 RBS
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only