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We narrowed to 74,014 results for: LAS

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Showing: 7641 - 7680 of 74014 results

  5. pcDNA 3.1-NbON10-NanoLuc

    Plasmid
    #198691
    Purpose
    Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture
    Depositor
    Gualberto González Sapienza
    Article
    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d
    Insert
    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    Tags
    6xHis tag, IgK leader sequence, NanoLuc, and twin…
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    198691-plasmid-map-sequence-id-402807

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  7. pUt-mNF-GFP

    Plasmid
    #199220
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199220-plasmid-map-sequence-id-396466

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  9. CBSH3- 3m shRNA3

    Plasmid
    #160213
    Purpose
    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    160213-plasmid-map-sequence-id-314924

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  10. CBSH3- 3m shRNA2

    Plasmid
    #160211
    Purpose
    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160211-plasmid-map-sequence-id-313962

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  11. CBSH3- 3m shRNA1

    Plasmid
    #160209
    Purpose
    Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160209-plasmid-map-sequence-id-313960

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  12. Rescue myc-PCDHGC5 mRNA

    Plasmid
    #122229
    Purpose
    Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.
    Depositor
    Angel de Blas
    Article
    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.
    Insert
    PCDHGC5 (Pcdhgc5 Rat)
    Tags
    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…
    Expression
    Mammalian
    Mutation
    Silent mutations are in five consecutive codons …
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122229-plasmid-map-sequence-id-237759

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  16. pFUSE2ss-CLIg-mK_M18

    Plasmid
    #82358
    Purpose
    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103
    Insert
    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    Tags
    none
    Expression
    Mammalian
    Promoter
    hEF1-HTLV prom
    Available Since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    82358-plasmid-map-sequence-id-400982

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  21. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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  25. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

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  28. BAP1 C91A

    Plasmid
    #81025
    Purpose
    Retroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection marker
    Depositor
    Anjana Rao
    Article
    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.
    Insert
    BAP1 (BAP1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    BAP1 C91A (point mutation, catalytically inactive)
    Promoter
    5’LTR
    Available Since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Promoter
    RPBSA and TRE promoter
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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  33. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid
    #91781
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Asp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91781-plasmid-map-sequence-id-181014

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  36. p1.1-mCherry

    Plasmid
    #162772
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    red fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162772-plasmid-map-sequence-id-339102

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  37. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid
    #91733
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91733-plasmid-map-sequence-id-180936

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  40. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

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Showing: 7641 - 7680 of 74014 results