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Showing: 9551 - 9600 of 23367 results

  6. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  12. AA391

    Plasmid
    #216033
    Purpose
    Fragmid fragment: (Cas protein) reverse TetR; binds to TRE in presence of doxycycline
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rTetR_v1.1
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216033-plasmid-map-sequence-id-431517

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  20. AA401

    Plasmid
    #215959
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215959-plasmid-map-sequence-id-431386

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  23. pYPQ262F-ABE8e

    Plasmid
    #199179
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30
    Insert
    ABE8e(V106W)-zSpCas9(D10A)
    Use
    CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    199179-plasmid-map-sequence-id-392966

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  24. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Article
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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Showing: 9551 - 9600 of 23367 results