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61,387 results

Showing: 9651 - 9700 of 61387 results

  1. pET-EIN-FTO(32-505)

    Plasmid
    #186466
    Purpose
    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    Fat mass obesity associated protein (FTO Human)
    Tags
    6 Histidine-EIN (N-terminal domain of E.coli Enzy…
    Expression
    Bacterial
    Mutation
    amino acid 32-505
    Promoter
    T7
    Available Since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186466-plasmid-map-sequence-id-365741

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  2. TFORF2660

    Plasmid
    #142350
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT1 (STAT1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142350-plasmid-map-sequence-id-397203

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  4. TFORF3200

    Plasmid
    #144676
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RUNX3 (RUNX3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144676-plasmid-map-sequence-id-350541

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  5. TFORF0623

    Plasmid
    #142666
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DDIT3 (DDIT3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142666-plasmid-map-sequence-id-397759

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  8. AICSDP-45: TTN-mEGFP

    Plasmid
    #114412
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114412-plasmid-map-sequence-id-220885

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  10. TFORF0158

    Plasmid
    #142469
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EWSR1 (EWSR1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142469-plasmid-map-sequence-id-397248

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  11. pSLIK sh human Rb 1534 hyg

    Plasmid
    #31500
    Depositor
    Julien Sage
    Insert
    RB shRNA (RB1 Human)
    Use
    Lentiviral and RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    The target sequence for shRB 1534 is GAACGATTATCC…
    Available Since
    Aug. 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    31500-plasmid-map-sequence-id-14427

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  12. FPR2-Tango

    Plasmid
    #66284
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    FPR2 (FPR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    66284-plasmid-map-sequence-id-263479

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  19. pcDNA3.1(+)GαoA-91-NLuc

    Plasmid
    #207151
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207151-plasmid-map-sequence-id-410283

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  20. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid
    #207152
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207152-plasmid-map-sequence-id-410279

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  23. pcDNA3-GFP-MMP7

    Plasmid
    #11989
    Depositor
    Steven Johnson
    Insert
    MMP7 (MMP7 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11989-plasmid-map-sequence-id-133484

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  24. MTNR1B-DuET

    Plasmid
    #213349
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MTNR1B (MTNR1B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213349-plasmid-map-sequence-id-430875

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  25. TFORF2547

    Plasmid
    #144039
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPD (CEBPD Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144039-plasmid-map-sequence-id-400533

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  27. TFORF2501

    Plasmid
    #142433
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PML (PML Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142433-plasmid-map-sequence-id-397227

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  30. pCI-Puro_hCOL4A4-Myc-His

    Plasmid
    #198093
    Purpose
    Express hCOL4A4 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A4 (COL4A4 Human)
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198093-plasmid-map-sequence-id-389398

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  31. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

    Plasmid
    #200726
    Purpose
    Lentiviral expression of Flag-Tagged human HEXIM1
    Depositor
    Claudio Alarcón
    Insert
    HEXIM1 (HEXIM1 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200726-plasmid-map-sequence-id-401797

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  33. Membrane mNeonGreen

    Plasmid
    #198057
    Purpose
    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Insert
    LCK membrane mNeonGreen (LCK A.victoria, Human)
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mNeonGreen
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198057-plasmid-map-sequence-id-389150

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  37. GPR12-Tango

    Plasmid
    #66309
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR12 (GPR12 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  42. pLV-Blast_hCOL4A5

    Plasmid
    #198100
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198100-plasmid-map-sequence-id-389263

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  49. TFORF2027

    Plasmid
    #143994
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NPAS2 (NPAS2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    143994-plasmid-map-sequence-id-430156

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  50. TFORF1066

    Plasmid
    #143919
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFATC2 (NFATC2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    143919-plasmid-map-sequence-id-401526

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Showing: 9651 - 9700 of 61387 results