We narrowed to 10,522 results for: SEC
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Plasmid#48118PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertsignal peptide of YngK
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
ninaE-dsocs
Plasmid#26857DepositorInsertSlow Termination of Phototransduction (stops Fly)
TagsmycExpressionInsectMutationThis STOPS construct contains a mutated/truncated…Available SinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-ElonginC
Plasmid#29505DepositorInsertElongin C (ELOC Human)
ExpressionInsectAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 CA
Plasmid#44830DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationQ70L (constitutive active)Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTL-Bag2-gRNA2
Plasmid#196252PurposePlasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAsDepositorInsertCRISPR-Cas9 guide RNA scaffold and multiple cloning site
ExpressionBacterialPromoterJ23119 promoterAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTL-Bag2T-gRNA2
Plasmid#196253PurposePlasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAsDepositorInsertCRISPR-Cas9 guide RNA scaffold and multiple cloning site
ExpressionBacterialPromoterJ23119 promoterAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRExpressionInsectAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-WT Split GFP
Plasmid#69891PurposeExpresses a GFP circular RNA in DrosophilaDepositorAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-fSFRP2-GFP
Plasmid#22915DepositorInsertfugu secreted frizzled receptor protein 2 promoter driving GFP
UseAAVExpressionMammalianAvailable SinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
Plasmid#169369PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
UseCRISPRExpressionInsectPromoterCPIJ039653Available SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159671PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertExuL-NLS-hSpCas9-T2A-GFP
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS
Plasmid#58325PurposeDestination vector for inserting ubi-CadTS to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-E3_5-HilA
Plasmid#153326PurposeTriggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-E3_5 DARPin
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp dsRed Rab5 CA
Plasmid#53467DepositorAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)
Plasmid#52907PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVMG0146_Cq-U6-1_2xBbsI-gRNA
Plasmid#169238PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertCq-U6-1_2xBbsI-gRNA
UseCRISPRExpressionInsectPromoterCPIJ039653Available SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0149_Cq-U6-6_2xBbsI-gRNA
Plasmid#169323PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0149_Cq-U6-6_2xBbsI-gRNA
UseCRISPRExpressionInsectPromoterCPIJ039596Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-K27-HilA
Plasmid#153328PurposeTriggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-K27 DARPin
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT2
Plasmid#118984PurposeMutational dissection of the G rich region of the c-kit promoterDepositorAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT4
Plasmid#118986PurposeMutational dissection of the G rich region of the c-kit promoterDepositorAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT6
Plasmid#118988PurposeMutational dissection of the G rich region of the c-kit promoterDepositorAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716Available SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-mCherry
Plasmid#102781PurposeBaculovirus rescue vector, VSVGED pseudotype, mCherryDepositorInsertmCherry
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab5 DN
Plasmid#44823DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS43N (dominant negative)Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS2381-Tier1-PhCMV-Igk_TM
Plasmid#169487PurposeTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).DepositorInsertPCMV-driven membrane localization
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Cquin_596
Plasmid#176669PurposeExpression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQUAST>stop>shibire (ts1)
Plasmid#24363DepositorInsertShibire (ts1) (shi Fly)
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
DYNLT1
Plasmid#132528PurposeHuman TCTEX-1, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 DN
Plasmid#44831DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS25N (dominant negative)Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP2 CC+CKK
Plasmid#59039Purposefor baculovirus expression of CAMSAP2 CC+CKK in Sf9 cellsDepositorAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 CA
Plasmid#44825DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationQ67L (constitutive active)Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-luc-TimRA
Plasmid#33133DepositorInsertTim-RA 3' UTR (tim Fly)
UseLuciferaseTagsFirefly LuciferaseExpressionInsectPromoterActin 5CAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAc-HT-Dmp53
Plasmid#85698Purposeexpression of Drosophila p53 in Drosophila cellsDepositorAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC+CKK
Plasmid#59048Purposefor baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)DepositorAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only