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FUW-TetO-Zeo-Ezh2

Plasmid

#40794

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_HOIP/RNF31_#6

Plasmid

#75252

Purpose

retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against human RNF31 (RNF31 )

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

Plasmid

#160046

Purpose

For expressing the full paradoxical circuit

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Inserts

osTIR1
iaaH
iCasp9
BlastR

Use

Synthetic Biology

Tags

mAID, mCherry, and mGFPmut3

Expression

Mammalian

Promoter

no promoter, T2A is used and pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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KA1042_pPB-hCMV*1-Esrrb-IV

Plasmid

#124177

Purpose

piggyBac-based Dox-inducible expression of Esrrb

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb-IRES-Venus (Esrrb Mouse)

Expression

Mammalian

Promoter

tetO-CMVmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Nsp10-mCherry

Plasmid

#165135

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp10 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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p51.SRE.mCMV.CD63-GFP.wpre

Plasmid

#226773

Purpose

CD63-GFP in p52 (SRE backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

CD63 (CD63 )

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE)

Plasmid

#214883

Purpose

Lentiviral vector encoding RfxCas13d targeting PURI guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPURI-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE)

Plasmid

#214884

Purpose

Lentiviral vector encoding RfxCas13d targeting GLY guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crGLY-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-ADA 6KQ

Plasmid

#17563

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 ADA 6KQ (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-AMPH1-BAR-mCherry

Plasmid

#85130

Purpose

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_Ntag_BbsI-Mammalian

Plasmid

#186674

Purpose

N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Mammalian N-terminal Tag

Tags

3xFlag-3xHA

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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plenti6.3/hCx43-stop

Plasmid

#27383

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Gja1 (GJA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pCC324

Plasmid

#110165

Purpose

miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

Depositor

Iva Greenwald

Article

de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

Insert

ERK-KTR-mClover-T2A-mCherry-H2B

Use

Minimos transposon

Tags

mCherry and mClover

Expression

Worm

Mutation

R61K change made in ERK-KTR CDS

Promoter

lin-31 promoter/enhancer

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Nsp7-EGFP

Plasmid

#165111

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_EF1a-NAE1_IRES_Puro

Plasmid

#235659

Purpose

human NAE1 cloned into into lentiviral backbone (pLV-EF1a-IRES-Puro)

Depositor

Lorenz Studer

Article

Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

Insert

NAE1 (NAE1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

Plasmid

#62953

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

Plasmid

#62952

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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2PH-PLCd-APEX2-P2A-mKate2

Plasmid

#71284

Purpose

Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLCd-APEX2-P2A-mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hTRF2

Plasmid

#103803

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)

Expression

Mammalian

Mutation

hTRF2: deletion of amino acids 1-42 and 476 compa…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mCherry

Plasmid

#209035

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA

Plasmid

#108941

Purpose

Mammalian expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9

Plasmid

#108942

Purpose

Mammalian expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTet-O-E2F7-T2A-PuroR

Plasmid

#162348

Purpose

Lentiviral expression of E2F7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

E2F7 (E2F7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPV25-cR-2iG

Plasmid

#204618

Purpose

Fluorescent reporter for SPI2 induction in Salmonella

Depositor

Jacques Neefjes

Article

Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

Inserts

rpsM promoter
sseA promoter

Tags

GFP and dsRed

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HER3-DMVF-181-R

Plasmid

#87621

Purpose

C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER3-DMVF181-C (ERBB3 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-mKdm2b (del CxxC domain)-Flag

Plasmid

#61740

Purpose

Express CxxC domain deleted mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Tags

Flag

Mutation

delete aa 588-624

Promoter

EF1a

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-LANP

Plasmid

#16175

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
Myc-Rab35 WT

Plasmid

#47433

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 (RAB35 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-mEmerald-VIM

Plasmid

#62849

Purpose

Donor vector for gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

mEmerald-VIM (VIM Synthetic, Human)

Use

Yeast-e.coli shuttle vector

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PLL5.0-Anillin ShRNA-Cherry-AnillinFL

Plasmid

#187271

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Promoter

U6, CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgSMARCA4 guide 1

Plasmid

#193604

Purpose

SMARCA4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSMARCA4 guide 1 (SMARCA4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SNAP-ACE2

Plasmid

#178592

Purpose

Expresses SNAP-fused ACE2 protein that can be fluorescently labelled using SNAP substrates.

Depositor

Ralf Jockers

Article

Cecon et al Cell Chem Biol. 2021 Jul 2. pii: S2451-9

Insert

angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Flag and SNAP

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-DMD-Donor

Plasmid

#60605

Purpose

Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

Hygromycin resistant gene

Use

Knock-in donor vector

Expression

Mammalian

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 FLAG DEPTOR (13×S/T->A)

Plasmid

#21702

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR 13xA (DEPTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

Nonphosphorylatable mutant. 13xA. Also has N204S …

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-WT-ATPIF1

Plasmid

#85403

Purpose

Retroviral vector expressing wild type human ATPIF1

Depositor

David Sabatini

Article

Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

Insert

ATPIF1 (ATP5IF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Wildtype

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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We narrowed to 64,611 results for: cell;