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162,536 results

Showing: 9901 - 9950 of 162536 results

  11. TFORF0208

    Plasmid
    #142556
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142556-plasmid-map-sequence-id-427312

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  13. mCherry-Vimentin-N-18

    Plasmid
    #55158
    Purpose
    Localization: Intermediate Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55158-plasmid-map-sequence-id-267740

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  14. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid
    #106174
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has Service
    AAV1 and AAV9
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184S
    Promoter
    hSynapsin
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106174-plasmid-map-sequence-id-204113

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  16. AAV-sCAG-mBaoJin-oPRE

    Plasmid
    #218684
    Purpose
    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mBaoJin
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Short variant of the CAG promoter
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218684-plasmid-map-sequence-id-438234

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  17. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  20. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Article
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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  28. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid
    #148120
    Purpose
    Mammalian Expression of HshnRNPH1
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPH1 (HNRNPH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148120-plasmid-map-sequence-id-396934

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  33. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

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  43. EBFP2-pBAD

    Plasmid
    #54542
    Purpose
    Localization: Protein Expression Vector , Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and EBFP2
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. pCAGGS_3E5E_eGFP

    Plasmid
    #103054
    Purpose
    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Article
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Insert
    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    Use
    Tags
    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    103054-plasmid-map-sequence-id-197128

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  47. pOpen-Bst HF

    Plasmid
    #165539
    Purpose
    High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst HF
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 1, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165539-plasmid-map-sequence-id-339129

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  50. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

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Showing: 9901 - 9950 of 162536 results