2,202 results
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Plasmid#113879Purposep3E entry vector for Three Multisite GatewayDepositorAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only
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pTol1-U6abc_tnnt2a_cmlc2-nmKate
Plasmid#238309PurposeDrives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.DepositorInsertnuclear mKate/3 gRNAs targeting tnnt2a
Promotercmlc2 (nmKate); U6 (gRNAs)Available SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
TagsmKate2ExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST mpeg1:tomato-CAAX
Plasmid#135215PurposeRed tagged macrophagesDepositorInsertmpeg1 and tdTomato
UseZebrafish expressionAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependent Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2
Plasmid#221712PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E ubb polyA
Plasmid#195977PurposeGateway p3E vectorDepositorInsertubb:polyA (ubb Zebrafish)
UseOtherAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab32b
Plasmid#140885PurposeExpression of zebrafish GFP-tagged Rab32b in mammalian cells.DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2DY-EF
Plasmid#221716PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationD651E and Y680F in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA
Plasmid#108871PurposeExpression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafishDepositorInserts5xU6:sgRNA
Cas9-t2A-GFP
UseCRISPRPromoterU6 and hsp70Available SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P--Dr-aCateninFL
Plasmid#191740PurposeExpresses FL D rerio catenin in bacteriaDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-0.349cldn15la
Plasmid#125026PurposeFive prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.DepositorInsertClaudin 15-like a (cldn15la Zebrafish)
Available SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E itga11a-cFos
Plasmid#240524Purpose5' Gateway Entry vector containing the itga11a-cFos promoter for fibroblast-specific expression in zebrafishDepositorInsertsUseUnspecifiedAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj14
Plasmid#173144PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj14 (kcnj14 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
MutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2_HA_dreAdar1
Plasmid#250540PurposeIn vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zebrafish Adar1DepositorInsertHA_dreAdar1
UseSynthetic BiologyAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tg(mpeg1:LifeAct-mRuby)
Plasmid#166977Purposelabels F-actin in macrophagesDepositorAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
TagsEGFPMutationY599H and I793S (please see depositor comment bel…Available SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk8
Plasmid#207615PurposeZebrafish Alk8 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Zebrafish, Vaucheria frigida)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9-T2A-GFP
Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayAvailable SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk3
Plasmid#207614PurposeZebrafish Alk3 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-BMPR2a
Plasmid#207616PurposeZebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOVDepositorInsertBMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Zebrafish, Vaucheria frigida)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementAvailable SinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9
Plasmid#63154PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLSDepositorInsertCas9
UseCRISPR; Tol2 middle entry vector for gatewayAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dat - EGFP - Tol2
Plasmid#85418Purposefor making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) geneDepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5E-smyhc1
Plasmid#126748PurposeZebrafish slow myosin heavy chain promoter. Clone 667DepositorInsertsmyhc1 (smyhc1 Zebrafish)
UseGateway CloningAvailable SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWO-SUMO-3C_14M_zTDP2
Plasmid#200512PurposeExpression of zebrafish TDP2 recombinant proteinDepositorInsertTDP2 (tdp2b Zebrafish)
TagsHIS-SUMOExpressionBacterialMutationL136N, A139S, C239T, K240R, K309Q, K314L, T315G, …Available SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-DRv7
Plasmid#78627PurposeGESTALT V7 barcode for zebrafishDepositorInsertGESTALT lineage tracing target V7
Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#72640PurposeGateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafishDepositorInsertelavl3 enhancer (elavl3 Zebrafish)
Available SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcryBFP_Umyo18bGFP
Plasmid#172527PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.DepositorInsertmyo18b
ExpressionMammalianAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCAC-69
Plasmid#31241PurposeMitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafishDepositorInsertmitochondrial localization enhanced green fluorescent protein
Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-PLIN2-tdTOMATO
Plasmid#172533PurposePLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloningDepositorInsertplin2-tdtomato
MutationS80R, A81S, E144D, I148F, and R415H mutations in …Available SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
TagsEGFPMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ubi:Zebrabow
Plasmid#118219PurposeFor ubiquitous expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-QF2
Plasmid#122563PurposeIntegration of QF2 sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-QF2-SV40pA
UseCloningAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c
Plasmid#107599PurposeAn entry vector with U6a and U6c promoter driving guide RNAs expressionDepositorInsertsU6a promoter
U6c promoter
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Budding Yeast, Neurospora crassa, Zebrafish)
UseZebrafish expressionPromoterhsp70lAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only