We narrowed to 1,383 results for: piggyBac
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Plasmid#214976PurposeFlag tagged LC3BDepositorInsertFLAG-LC3B (MAP1LC3B Human)
ExpressionMammalianAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyPromoterhPGK promoterAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster vasaAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKH152_PB_flag-LC3B_LDSmut_IRES-BFP
Plasmid#214977PurposeFlag tagged LDS mutant LC3BDepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI-Luc
Plasmid#133364PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
TagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL005635 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-REEP5
Plasmid#201928PurposePiggyBac Vector expressing KEIMA-REEP5DepositorInsertREEP5 (REEP5 Human)
ExpressionMammalianAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-AgDD
Plasmid#80625Purposecreate nuclear protein aggregates in mammalian cellsDepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC150-TEX264-GFP
Plasmid#201931PurposePiggyBac Vector expressing TEX264-GFPDepositorInsertTEX264 (TEX264 Human)
ExpressionMammalianAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
EK0379 CMV-TO-EGFP-stop-t70587 (PB)
Plasmid#191154PurposeExpression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.DepositorInsertEGFP-t70587
ExpressionMammalianMutationWTPromoterCMV-TOAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC150-RFP-EGFP-LGALS3 R186S
Plasmid#175779PurposePiggyBac Vector expressing GFP-RFP-LGALS3 R186SDepositorAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO deleted ITR
Plasmid#67906PurposePiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeatDepositorInsertNeoR (neoR Synthetic)
ExpressionMammalianAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-Puro
Plasmid#115143PurposeSafe harbor site 231 knock-in vector with puromycin resistance selection cassetteDepositorInsertPuromycin
ExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo
Plasmid#102885PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster nanosAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast
Plasmid#205552PurposeExpress EPN24-MCP-p6-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP-p6
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-BGHpA
Plasmid#92161PurposeFor lncRNA overexpression (in trans)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-IGF2
Plasmid#133374PurposePiggybac vector for constitutive expressionDepositorInsertIGF2 (IGF2 Human)
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6c
Plasmid#117223PurposeExpresses gRNA in Aedes aegyptiDepositorInsertHR U6c promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6b
Plasmid#117222PurposeExpresses gRNA in Aedes aegyptiDepositorInsertHR U6b promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-ASCL2
Plasmid#133375PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChiNW-ASCL2
Plasmid#133384PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH
Plasmid#179586PurposeePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)DepositorInsertNLS-NG-T2A-hSHH
UsePiggybacTagsNeonGreenPromoterTREAvailable SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAEL006511-Cas9
Plasmid#100580PurposeThe promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL006511-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL006511 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6c-T
Plasmid#117211PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6c promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS YA
Plasmid#140012PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126APromoterEF1Available SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
ExpressionMammalianAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT(UUA)_FLAG-AcKRS
Plasmid#140010PurposePlasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAEL007097-Cas9
Plasmid#100705PurposeThe promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL007097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-BtW-eSpCas9
Plasmid#133358PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
TagsFlag-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAEL003877-Cas9
Plasmid#100581PurposeThe promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL003877-hspcas9-GFP
Opie-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL003877 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA
Plasmid#172226PurposeExpression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.DepositorInsertSOX17 (SOX17 Human)
UsePiggybac transposonTags3XFLAG, DD-domain, GS-linker, and TagBFPExpressionMammalianPromoterCAGAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6b-T
Plasmid#117210PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6b promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS
Plasmid#140013PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA
Plasmid#140014PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126A, Y197FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0510
Plasmid#124001PurposeEncodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast
Plasmid#205547PurposeExpress EPN11-MCP-I-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN11-MCP-I
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast
Plasmid#205548PurposeExpress EPN11-MCP-p6-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN11-MCP-p6
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PtW-eSpCas9
Plasmid#133357PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
TagsHA-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0512
Plasmid#124003PurposeEncodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_002
Plasmid#123960PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - 2xHS4 - HygroR
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0511
Plasmid#124002PurposeEncodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_phubc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0520
Plasmid#124011PurposeEncodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pCMV-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA
Plasmid#154779Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertLaG17-SynNotch
TagsLaG17ExpressionMammalianMutation204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAEL007584-Cas9
Plasmid#100706PurposeThe promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007584-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL007584 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT A41AA C55A sfGFP 150TAG
Plasmid#154770Purposeplasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, G1 PylT with A41AA and C5…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6a-T
Plasmid#117209PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6a promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8b_001
Plasmid#123890PurposeEncodes the 5' PiggyBac transposon recognition site cassette as a type 8b part to be used in the MTK systemDepositorInsert5'PB
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK7_001
Plasmid#123870PurposeEncodes the 3' PiggyBac transposon recognition site cassette as a type 7 part to be used in the MTK systemDepositorInsert3'PB
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only