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We narrowed to 15,858 results for: cas9

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Showing: 381 - 400 of 15858 results

  2. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid
    #101209
    Purpose
    Bacterial expression plasmid for SpCas9-HF1 variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101209-plasmid-map-sequence-id-198127

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  4. px459 VQR

    Plasmid
    #101715
    Purpose
    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q and T1337R)
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101715-plasmid-map-sequence-id-196971

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  5. PX852

    Plasmid
    #62884
    Purpose
    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62884-plasmid-map-sequence-id-105906

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  7. PX854

    Plasmid
    #62886
    Purpose
    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa573-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62886-plasmid-map-sequence-id-105909

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  9. PB_tre_dCas9_KRAB

    Plasmid
    #126030
    Purpose
    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion
    Depositor
    Mauro Calabrese
    Publication
    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9 fusion with KRAB
    Hygromycin resistance
    Use
    CRISPR; Piggybac
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    EF1 alpha and TRE
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126030-plasmid-map-sequence-id-245793

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  11. DS-NMcas

    Plasmid
    #48646
    Purpose
    Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    NM tracrRNA
    Use
    CRISPR
    Promoter
    NM tracrRNA promoter and proC
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48646-plasmid-map-sequence-id-71690

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  13. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47948-plasmid-map-sequence-id-68812

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  15. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6b
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207852-plasmid-map-sequence-id-408170

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  20. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid
    #108570
    Purpose
    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi
    Insert
    dSpyCas9
    Use
    CRISPR and Lentiviral
    Tags
    APEX2, Destabilization domains, Flag, and mCherry…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108570-plasmid-map-sequence-id-328916

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Showing: 381 - 400 of 15858 results