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We narrowed to 14,327 results for: cas9

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Showing: 381 - 400 of 14327 results

  1. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Article
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Promoter
    sp6
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

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  2. pSpCas9(BB)-2A-Blast

    Plasmid
    #118055
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118055-plasmid-map-sequence-id-443898

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  14. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  15. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

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  19. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Promoter
    constitutive 35SPPDK promoter
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52256-plasmid-map-sequence-id-116013

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Showing: 381 - 400 of 14327 results