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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

Plasmid

#199604

Purpose

Expression of cell permeable spCas9 in E. Coli.

Depositor

Junwei Shi

Publication

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

Cas9-T6N

Use

CRISPR

Expression

Bacterial

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-EGFP-IRES-puro

Plasmid

#128652

Purpose

mammalian expression of EGFP with puromycin selection

Depositor

Robert Sobol

Publication

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1(+)-ExRai-AKAR2

Plasmid

#161753

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

Depositor

Jin Zhang

Publication

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ExRai-AKAR2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Publication

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-Puro

Plasmid

#141388

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf7a (ORF7a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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Flag-TRIM28

Plasmid

#124960

Purpose

Mammalian expression of Human TRIM28

Depositor

Michelle Barton

Publication

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

FLAG

Expression

Mammalian

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDEST-CMV-3xFLAG-gateway-EGFP

Plasmid

#122845

Purpose

N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Publication

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FKBP-V5-AP-nes_pLX304

Plasmid

#120912

Purpose

Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

FKBP-V5-AP-NES

Use

Lentiviral

Expression

Mammalian

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lenti-SpCas9 neo

Plasmid

#104996

Purpose

This lentiviral construct delivers hSpCas9 and G418 resistance.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUCM-CLYBL-hNIL

Plasmid

#105841

Purpose

Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

Depositor

Michael Ward

Publication

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCDH-puro-EWS-FLI1

Plasmid

#102813

Purpose

Overexpress EWS-FLI1. 3rd generation lentiviral vector.

Depositor

Jialiang Wang

Publication

Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

Insert

EWS-FLI1 (EWSR1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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STARR-seq luciferase validation vector_ORI_empty

Plasmid

#99297

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Publication

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has service

AAV1, AAV2, AAV5, AAV9, and AAV Retrograde

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc. Inducible with tetracycline or doxycycline.

Depositor

Takuji Iwasato

Publication

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Promoter

EF-1 alpha

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDH-puro-CMV-VC3AI

Plasmid

#78907

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Publication

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

VC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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