4,142 results

1. pCMV5B-TGFbeta receptor I K232R

   Plasmid

   #11763

   Depositor

   Jeff Wrana

   Article

   Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

   Insert

   TGFbeta receptor I (TGFBR1 Human)
   

   Use

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   K232R: kinase dead

   Promoter

   CMV

   Available since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMXs-Sox2-IP

   Plasmid

   #15919

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   SRY-box containing gene 2 (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1014 pcDNA3 T7 Akt1 K179M T308A S473A

   Plasmid

   #9031

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

   Plasmid

   #40131

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease (KKR)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. EdTC

   Plasmid

   #24310

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   31 first amino acids of hTcf4 have been deleted.

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEX-SP-YFP-STIM1(23-685)

   Plasmid

   #18857

   Depositor

   Tobias Meyer

   Article

   Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

   Insert

   Stromal interaction molecule 1 (STIM1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   YFP is inserted between residues 1-23 (signal pep…

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-CIBN-CreC

   Plasmid

   #26889

   Purpose

   Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIBN-CreC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 1-170 of CIB1, aa 106-343 of Cre

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDS221

   Plasmid

   #34981

   Purpose

   Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb1–mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMLM3705

   Plasmid

   #47754

   Purpose

   Expresses mammalian cell codon-optimized dCas9-VP64

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   codon optimized dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9 (catalytically i…

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1319 pcDNA3 flag FKHRL1 AAA

    Plasmid

    #10709

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHRL1 AAA (FOXO3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    AAA mutant (see map) - T32A, S253A, S315A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RIP Cre-ER (DM#265)

    Plasmid

    #15029

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    Rat insulin promoter (RIP) (Ins2 Rat)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Cre-ER (TM)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pARE-lux

    Plasmid

    #11768

    Depositor

    Joan Massague, Jeff Wrana

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    ARE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pRsetB-his7-Perceval

    Plasmid

    #20336

    Depositor

    Gary Yellen

    Article

    Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

    Insert

    Perceval (fluorescent ATP:ADP sensor)
    

    Use

    Tags

    his(7)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGMKOSiE

    Plasmid

    #20865

    Depositor

    Keisuke Kaji

    Article

    Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

    Insert

    myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2443

    Plasmid

    #72252

    Purpose

    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF-ENTR A (696-6)

    Plasmid

    #17427

    Purpose

    Gateway entry vector with EF1-a promoter, V5-His C-term, frame A

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 barr2 HA

    Plasmid

    #14692

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta-arrestin 2 (Arrb2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAlbumin

    Plasmid

    #22037

    Depositor

    Didier Trono

    Insert

    partial sequence of human albumine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2-hDKK1-flag

    Plasmid

    #15494

    Depositor

    Sergei Sokol

    Article

    Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

    Insert

    DKK1 (DKK1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

20. SRa-HA-SUMO2 (Sentrin 3)

    Plasmid

    #17360

    Purpose

    Mammalian expression of human SUMO2 with HA tag

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge