We narrowed to 54,283 results for: Ran

1. ECFP HO

   Plasmid

   #207413

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ECFP CD

   Plasmid

   #207414

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB533-44B12m23-sfGFP

   Plasmid

   #186778

   Purpose

   RNAP2 Ser5ph-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   44B12m23-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mRuby2-ZO1-C-14

   Plasmid

   #55916

   Purpose

   Localization: Tight Junctions, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   ZO1 (TJP1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. Fragment1 with TLS2

   Plasmid

   #196983

   Purpose

   Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Intermediate fragment for assembly of gRNA-TLS2 construct
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Perturb-FISH

   Plasmid

   #220626

   Purpose

   expression of gRNAs under U6T7 promoter

   Depositor

   Samouil Farhi

   Article

   Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BsmBI

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPagP

   Plasmid

   #112125

   Purpose

   Bacterial expression of outer membrane protein PagP, wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   PagP (pagP E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-N2-COL1A1

   Plasmid

   #66602

   Purpose

   Expresses procollagen 1 alpha 1 as a GFP fusion

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

   Insert

   COL1A1 (COL1A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX_DSB_Spectrum-V1

   Plasmid

   #192474

   Purpose

   Lentiviral DSB Spectrum V1 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. amilCP chromoprotein

    Plasmid

    #117847

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

    Plasmid

    #60629

    Purpose

    Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP64 human MyoD (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2-MT GLI2 FL

    Plasmid

    #17648

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Use

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. phage UBC NLS-HA-2XMCP-tagRFPt

    Plasmid

    #64541

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    HA, NLS, and tagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX_DSB_Spectrum-V3

    Plasmid

    #192476

    Purpose

    Lentiviral DSB Spectrum V3 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, mCherry, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. phage ubc nls ha pcp gfp

    Plasmid

    #64539

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    EGFP, FactorXa site, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBMN(CMV-copGFP-Luc2-Puro)

    Plasmid

    #80389

    Purpose

    To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Luc2-F2A-Puromycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcdna nesprin TS

    Plasmid

    #68127

    Purpose

    nesprin tension sensor

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    mouse nesprin mini 2G (Syne2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. amilCP chromoprotein promoter-less

    Plasmid

    #118008

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUAS-V5-TurboID-NES

    Plasmid

    #116904

    Purpose

    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Arl13b-mcherry

    Plasmid

    #234845

    Purpose

    A mammalian expression plasmid encoding Arl13b with mcherry at C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Arl13b (ARL13B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CD8a-EGFP

    Plasmid

    #86051

    Purpose

    A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BFP-Sec61 beta

    Plasmid

    #49154

    Purpose

    expression of Sec61 beta fused to BFP, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUAS-V5-miniTurboID-NES

    Plasmid

    #116905

    Purpose

    Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurboID
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mRuby2-SiT-N-15

    Plasmid

    #55912

    Purpose

    Localization: Golgi TGN, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pLenti-CMV-cGAS-HA

    Plasmid

    #130910

    Purpose

    Expresses human cGAS-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge