165,075 results

1. VPS13B^Halo

   Plasmid

   #224584

   Purpose

   codon optimized Halo tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^Halo (VPS13B Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe_puro_DEST_Flag_SKIL

   Plasmid

   #45265

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   SNON (SKIL Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCap-SG5-Hgy

   Plasmid

   #227580

   Purpose

   Capture plasmid with hygromycin resistance and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GPR171-DuET

   Plasmid

   #213269

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR171 (GPR171 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209653

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

   Plasmid

   #71878

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC-delta (Otc Rat)
   

   Mutation

   Deleted amino acid 30-114 from full length OTC

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307 GFP-Kif26b-C

   Plasmid

   #118483

   Purpose

   lentiviral expression of GFP-Kif26b-C in mammalian cells

   Depositor

   Henry Ho

   Article

   Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

   Insert

   mouse Kif26b-C (Kif26b Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Encodes GFP fused to amino acid 1735 to 2112 of m…

   Promoter

   EF1A

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p667-UBC-NEK7-V5-miniTurbo_IDG-K

   Plasmid

   #189882

   Purpose

   Gateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   NEK7 (NEK7 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB1_hMSH6

    Plasmid

    #129424

    Purpose

    Expression human MSH6 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6 (MSH6 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-syn-dLight1.3b

    Plasmid

    #135762

    Purpose

    For expression of the genetically encoded dopamine sensor dLight1.3b

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLD-puro-MTS-EGFP

    Plasmid

    #190270

    Purpose

    Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

    Depositor

    Christian Münch

    Article

    Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

    Insert

    MTS-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. evoBE4-SpRY

    Plasmid

    #226854

    Purpose

    Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-on circRNA Expression Vector

    Plasmid

    #92351

    Purpose

    The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

    Depositor

    Ling-Ling Chen

    Article

    Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

    Insert

    mCherry and split EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTet

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pROF051

    Plasmid

    #155328

    Purpose

    Constitutive expression of SRDX-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-SRDX-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-SFFV-GFP1-10

    Plasmid

    #80409

    Purpose

    Expresses GFP1-10 in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST40-Arl13b89

    Plasmid

    #40874

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-PIG-empty

    Plasmid

    #105594

    Purpose

    empty control vector with GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    no insert, empty control vector
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV-LTR

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMo130-TelR

    Plasmid

    #50799

    Purpose

    Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

    Depositor

    Kim Lee Chua

    Article

    Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

    Insert

    tellurite-resistance cassette
    

    Use

    Suicide vector

    Promoter

    PCs12

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CAG-miRFP720

    Plasmid

    #197213

    Purpose

    Expresses the protein of miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    AAV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. T7-p73DD-pcDNA3

    Plasmid

    #22978

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p73 (TP73 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 327-…

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. pKAR2-Br512

    Plasmid

    #161875

    Purpose

    Expresses Br512 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Maranhao et al medRxiv

    Insert

    Modified DNA Polymerase I Large Fragment (Br512)
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Promoter

    T7, tetPA

    Available Since

    Nov. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. mCitrine-Farnesyl-5

    Plasmid

    #56558

    Purpose

    Localization: Membrane, Excitation: 516, Emission: 529

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

26. p663-UBC-miniTurbo-V5-MARK4_IDG-K

    Plasmid

    #189884

    Purpose

    Gateway destination clone of MARK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MARK4 (MARK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-jAspSnFR3

    Plasmid

    #203488

    Purpose

    jAspSnFR3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    jAspSnFR3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TMED4-bio-His

    Plasmid

    #51854

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED4 (TMED4 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLV-PGK-DIO-mCherry-nega miR

    Plasmid

    #137732

    Purpose

    Lentivirus vector for control miR in a Cre-dependent manner

    Depositor

    Tomoyuki Furuyashiki

    Article

    Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

    Insert

    control microRNA
    

    Use

    Lentiviral

    Tags

    mChherry

    Expression

    Mammalian

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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