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We narrowed to 12,455 results for: cel.2;

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Showing: 10151 - 10200 of 12455 results

  2. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Available Since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  13. pODN-mNG-ECT2

    Plasmid
    #183835
    Purpose
    Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183835-plasmid-map-sequence-id-373473

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  16. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

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  17. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

    Plasmid
    #49064
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49064-plasmid-map-sequence-id-72188

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  18. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid
    #49070
    Purpose
    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    ECL4 deletion of amino acids 562-582 in hNKCC1 in…
    Promoter
    CMV
    Available Since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49070-plasmid-map-sequence-id-72197

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  37. plRL19

    Plasmid
    #163634
    Purpose
    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Insert
    L5 Integrase
    Expression
    Bacterial
    Promoter
    Mycobacterial Optimized Promoter (MOP) and Tet-On
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163634-plasmid-map-sequence-id-339549

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  38. plRL58

    Plasmid
    #166886
    Purpose
    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Tet-On
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    166886-plasmid-map-sequence-id-339556

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  39. plRL61

    Plasmid
    #163633
    Purpose
    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Tet-On
    Available Since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163633-plasmid-map-sequence-id-339483

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  46. pCiPS-NIFV

    Plasmid
    #110807
    Purpose
    Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells
    Depositor
    David Russell
    Article
    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10
    Insert
    Pol
    Expression
    Mammalian
    Mutation
    2 mutations in the DDE catalyic core motif of the…
    Promoter
    CMV
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110807-plasmid-map-sequence-id-222708

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Showing: 10151 - 10200 of 12455 results