We narrowed to 9,308 results for: control

1. pRSFDuet-1-Cdc45

   Plasmid

   #208796

   Purpose

   Xenopus laevis FLAG5-His10-TEV-Cdc45

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   Cdc45 (cdc45.L Frog)
   

   Tags

   FLAG and His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLA2ScIfmTcIfm

   Plasmid

   #193792

   Purpose

   Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLLacO-1 and PLTetO-1

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

   Plasmid

   #194973

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform C4a) (Gphn Rat, Synthetic)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

   Plasmid

   #194975

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform C4d) (Gphn Rat, Synthetic)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSyn-fDIO-mEGFP-Gphn_P1

   Plasmid

   #194978

   Purpose

   AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)
   

   Use

   AAV

   Tags

   mEGFP

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-fDIO-mScarlet-Gphn_P1

   Plasmid

   #194972

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform P1) (Gphn Rat, Synthetic)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

   Plasmid

   #194974

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform C4c) (Gphn Rat, Synthetic)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

   Plasmid

   #191651

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBeast-pT7-lox

   Plasmid

   #190059

   Purpose

   Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   lox
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBeast-pT7-codA

    Plasmid

    #190058

    Purpose

    Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    codA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBeast-pYjjZ-RBS- sfGFP

    Plasmid

    #190054

    Purpose

    GFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBeast-pKatG-RBS- sfGFP

    Plasmid

    #190053

    Purpose

    GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBeast-pOxyS-RBS- sfGFP

    Plasmid

    #190052

    Purpose

    GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPhiC31-actIIORF4

    Plasmid

    #190785

    Purpose

    The PhiC31-phage based integration vector containing the actII-ORF4 gene under the control of strong kasOp* promoter

    Depositor

    Jan Kormanec

    Article

    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

    Inserts

    T5 terminator
    actII-4
    kasOp*
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid

    #186413

    Purpose

    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli)
    

    Use

    Gateway destination vector

    Tags

    Nuclear localization signal

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

    Plasmid

    #168252

    Purpose

    "label active Rac; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    PBD GFP
    

    Use

    Zebrafish expression

    Promoter

    LyzC

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

    Plasmid

    #168253

    Purpose

    "label stable F-actin; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Utrophin-GFP
    

    Use

    Zebrafish expression

    Promoter

    LyzC

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L2_2xNOP1gRNA-Cas9-CsA

    Plasmid

    #136140

    Purpose

    Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid

    #173211

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

    Plasmid

    #173212

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

    Plasmid

    #173213

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV.U6gLacZ.EFS-NS.H2B-RFP

    Plasmid

    #170363

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Promoter

    EFS-NS

    Available Since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLEX305-N-dTAG-CARD8-ZUC

    Plasmid

    #169990

    Purpose

    Mammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    dTAG

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. dTAG NLRP1 P1214R

    Plasmid

    #166826

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Expression

    Mammalian

    Mutation

    P1214R

    Promoter

    hPGK

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p8115 LentiCRISPR v2 sgPTPN14-3

    Plasmid

    #163314

    Purpose

    Expresses Cas9 and a human PTPN14-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgPTPN14-3
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAA2

    Plasmid

    #154162

    Purpose

    Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Mutation

    16 wt NF-κB sites were replaced with Rel1A-specif…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAA1

    Plasmid

    #154161

    Purpose

    Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Mutation

    12 wt NF-κB sites were replaced with Rel1A-specif…

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKM62

    Plasmid

    #154160

    Purpose

    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Mutation

    4 wt NF-κB sites were replaced with Rel2-specific…

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5-FRT-TO-Orf6

    Plasmid

    #157726

    Purpose

    mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Orf6 (ORF6 SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pcDNA5-FRT-TO-Nsp15

    Plasmid

    #157720

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp15 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA5-FRT-TO-Nsp14

    Plasmid

    #157717

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp14 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. gamma2 CR3m shRNA

    Plasmid

    #120795

    Purpose

    Negative control shRNA for plasmid 120794: shRNA targeting the coding region of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. gamma2 UTR3m shRNA

    Plasmid

    #120797

    Purpose

    Negative control shRNA for plasmid 120796: shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCR2.1-Ins2-842

    Plasmid

    #53969

    Purpose

    842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

    Depositor

    Kevin Ferreri

    Article

    Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

    Insert

    mouse Ins2 gene partial (Ins2 Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGMEH-0X750-luxAB-DAS+4

    Plasmid

    #49519

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Promoter

    P750

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. DCT.VV.Orf135

    Plasmid

    #12823

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf135
    

    Expression

    Mammalian

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

37. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

    Plasmid

    #170222

    Purpose

    Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Mutation

    R24C

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSI-Check2-hRluc-NFkB-firefly

    Plasmid

    #106979

    Purpose

    Luciferase reporter with internal control for NFkB signal.

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    NFKB recognition sites (NFKB1 Human)
    

    Use

    Luciferase

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge