We narrowed to 9,773 results for: CAG

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JEH127

Plasmid

#179299

Purpose

Expresses dCas9 fused to VPR

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VPR

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-MYC

Plasmid

#242789

Purpose

Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-MYC in the other direction.

Depositor

Andrew Payne

Inserts

mNeonGreen
tTA-Advanced

Use

AAV

Tags

MYC

Promoter

TRE and hSyn

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW281_dSpRY

Plasmid

#244822

Purpose

Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CAG

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-GZV-SynZiFTR_ETV2_Down-Tandem

Plasmid

#241394

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of ETV2

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

ETV2; ZF10-NS3-p65 (ETV2, RELA, KRAS Human)

Expression

Mammalian

Promoter

ZF10; CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSB-XPRESSO-CaViar

Plasmid

#237299

Purpose

Sleeping Beauty XPRESSO vector for CaViar expression

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

CaViar

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pPB.DEST-ITGB1(WT)-mRuby2

Plasmid

#215448

Purpose

Piggybac expression vector with integrin beta1 tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

Plasmid

#229795

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-Flex-MCS-WPRE-SV40 - GCH14

Plasmid

#230821

Purpose

AAV cloning vector for CRE-dependent expression under the EF1a promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV and Cre/Lox; Cloning vector

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pB-3xFlag-dCas13-mTET2HxDCD

Plasmid

#228235

Purpose

For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

Depositor

Chuan He

Article

Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

Inserts

dCas13
TET2 (Tet2 Mouse)

Use

CRISPR

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Promoter

CAG

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGag-mCherry

Plasmid

#212631

Purpose

Expression vector for an MLV Gag-mCherry fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.16

Plasmid

#212532

Purpose

Encodes SARS-CoV-2 variant XBB.1.16 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1.16 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.5

Plasmid

#212523

Purpose

Encodes SARS-CoV-2 variant XBB.1.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin XLone-eGFP

Plasmid

#159754

Purpose

AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

all-in-one tet-on system

Use

CRISPR and TALEN; Donor plasmid for targeted knoc…

Expression

Mammalian

Promoter

TRE3GS inducible promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCH62 (denAsCas12a-KRAB piggyBac)

Plasmid

#217333

Purpose

piggyBac expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

D908A/E174R/S542R/K548R

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-Mito-DsRed

Plasmid

#210328

Purpose

Overexpression of Mito-DsRed

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

Mito-DsRed

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-GFP-hMyo19 iso2

Plasmid

#210333

Purpose

Overexpression of hMyo19

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

hMyo19 (MYO19 Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCXLE-Sox2-T2A-Klf4-E2A-Myc

Plasmid

#210018

Purpose

episomal SKM (expressing mouse Sox2 + Klf4 + Myc) for integration-free naïve reset

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pF hPGK mc2 IRES hygro

Plasmid

#206238

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-iRFP670-P2A-EGFP

Plasmid

#198060

Purpose

The lentiviral vector for iRFP670 and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP670

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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