159,238 results
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Plasmid#32078DepositorTypeEmpty backboneUseGateway destination binary vectorTagsHA-Prescission-BiotinExpressionMutationPromoter35S promoterAvailable sinceDec. 6, 2011AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2
Plasmid#194680PurposeCMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2DepositorInsertHpep1-betaArrestin2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF1066
Plasmid#143919PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFATC2 (NFATC2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV8-hSyn-flex-miR30-eGFP-shDyn
Plasmid#132716PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphinDepositorInsertGFP-shDyn (Pdyn Rat)
UseAAVTagsExpressionMutationPromoterSynapsin 1Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP
Plasmid#194683PurposeCMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_05-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Fibrillarin
Plasmid#26673DepositorInsertFibrillarin (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI Neo mouse PECAM FL
Plasmid#42901DepositorInsertPECAM (Pecam1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin
Plasmid#187957PurposeFKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-KRAB-SpdCas9-tagBFP
UseCRISPR and Synthetic BiologyTagsGGSExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME pHluorin2
Plasmid#73794Purposeratiometric pHluorin2 sequence in a middle entry cloneDepositorInsertratiometric pHluorin2
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa K44A
Plasmid#22197DepositorInserthuman Dynamin 1aa K44A (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_FRT_GFP
Plasmid#127108DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-q ONE-GO
Plasmid#189738PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6f
Plasmid#207856PurposeMammalian expression of PE6f prime editorDepositorInsertPE6f
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cTNT.iCre
Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorInsertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…ExpressionMutationPromoterChicken cardiac troponin TAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-OSBP-PH
Plasmid#49571Purposeexpresses the plekstrin homology domain of OSBP in mammalian cellsDepositorInsertOSBP-PH (AA 91-179) (OSBP Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KMT2A_MLLT1
Plasmid#205842PurposeExpress mEGFP-tagged fusion protein, KMT2A_MLLT1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-SNAP
Plasmid#38012DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsSNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbAW4k-loxP-TT-loxP-mRFP1
Plasmid#134405PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.DepositorInsertW4-loxP-TT-loxP-mRFP1
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterW4 (modified T7A1)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd nog
Plasmid#115488PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Progerin
Plasmid#69061Purposeencodes the lamin A mutant with a 50 amino acids deletion responsible for the Hutchinson-Gilford Progeria SyndromeDepositorInsertProgerin (LMNA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM4D(Gi)
Plasmid#45548PurposeExpresses hM4D (Gi) neuronal inhibitorDepositorInserthM4D (Gi)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
MS2-p65-HSF1-P2A-mCherry
Plasmid#184440PurposeMS2 coat protein fused with p65 and hsf1DepositorInsertMS2-p65-hsf1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1 hygro mCherry
Plasmid#128057PurposeMammalian expression vector with N terminal mCherryDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRGEB33
Plasmid#158151PurposeConstruction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-dnMCAK
Plasmid#205993PurposeOver-expression of GFP-dnMCAKDepositorInsertdnMCAK
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPK605
Plasmid#38148DepositorInsertsunc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
UseTagsExpressionWormMutationPromoterpie-1 promoter and unc-119Available sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Alpha-5-Integrin-12
Plasmid#54970PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertAlpha-5 Integrin (ITGA5 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CXCR6-DuET
Plasmid#213223PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR6 (CXCR6 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoter7SL upstream pollII enhancerAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJW1310
Plasmid#61253PurposeTemplate for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusionDepositorInsertU6 promoter
UseCRISPR; Cloning templateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-CD151-7
Plasmid#54873PurposeLocalization: Tetraspanin/Membrane, Excitation: 568, Emission: 592DepositorInsertCD151 (CD151 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ER-RA
Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddRFP A
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBlueSKMimRNA
Plasmid#102535PurposeTemplate for synthesis of Minos transposase mRNADepositorInsertMinos transposase mRNA
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_hACE2_PURO
Plasmid#155295PurposeLentiviral vector to generate hACE2 stable expressing cell lineDepositorInserthACE2 (ACE2 Human)
UseLentiviralTagsC9ExpressionMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3-Flag MKK6
Plasmid#13517DepositorInsertMKK6 (MAP2K6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only