We narrowed to 1,554 results for: ICL

1. pJL1-sfGFP-MOOR

   Plasmid

   #198212

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_MOOR
   

   Use

   Tags

   6x His Tag and MOOR glycosylation tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP-TPD54

   Plasmid

   #172448

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

   Insert

   TPD54 (TPD52L2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-VSP4-E228Q

   Plasmid

   #80351

   Purpose

   Expression of GFP-tagged, dominant negative VPS4 mutant

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   VPS4-E228Q (VPS4A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac HT JS-Munc18c

   Plasmid

   #135555

   Purpose

   Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18c
   

   Use

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-FLAG-Bbs4

   Plasmid

   #128868

   Purpose

   For mammalian expression of mouse Bbs4

   Depositor

   Aimin Liu

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

   Insert

   Bbs4 (Bbs4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-fLuc=tdT

   Plasmid

   #120870

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   pcDNA3-fLuc=tdT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSFDuet-1_hGH

   Plasmid

   #182410

   Purpose

   Bacterial expression of human growth hormone

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   hGH (GH1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5-HA-Parkin

   Plasmid

   #17613

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. human oct4-GFP

   Plasmid

   #21153

   Depositor

   Rudolf Jaenisch

   Article

   Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

   Insert

   hOCT4-GFP (POU5F1 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR-HLA-A2_GFP

    Plasmid

    #85161

    Purpose

    HLA.A2_GFP fusion with 2 glycines in between

    Depositor

    Tone Fredsvik Gregers, Sébastien Walchli

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Gateway entry vector

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-Aka-P2A-GFP

    Plasmid

    #120867

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insert Akaluc and a P2A site

    Promoter

    CAG Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FRB-mCherry-Sec61β

    Plasmid

    #186574

    Purpose

    Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    SEC61B (SEC61B Human)
    

    Use

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. σ3-mCherry

    Plasmid

    #186580

    Purpose

    Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP3S1 (AP3S1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMEH371 CD63 HA

    Plasmid

    #168220

    Purpose

    Mammalian expression of tetraspanin CD63 fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-TPD54

    Plasmid

    #172449

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 FlipGFP(Mpro) T2A mCherry

    Plasmid

    #163078

    Purpose

    Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

    Depositor

    Xiaokun Shu

    Article

    Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

    Insert

    FlipGFP-based Mpro activity reporter and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFPC1-Cep83

    Plasmid

    #128874

    Purpose

    For mammalian expression of mouse Cep83

    Depositor

    Aimin Liu

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

    Insert

    Cep83 (Ccdc41 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBa.Halo-myosin Va tail 1006-1828

    Plasmid

    #162715

    Purpose

    Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Va (Myo5a Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1005 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBa.GFP-myosin Vb tail 1436-1846

    Plasmid

    #162714

    Purpose

    Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Vb (Myo5b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1435 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-HiBiT-CD81

    Plasmid

    #162593

    Purpose

    N-terminally HiBiT-tagged human CD81 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD81 (CD81 Synthetic, human, Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCIpA102-G-HLA-A2_GFP

    Plasmid

    #85162

    Purpose

    for mRNA expression of HLA.A2-EGFP

    Depositor

    Tone Fredsvik Gregers, Sébastien Walchli

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Mrna expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2-HsProm1s1-mScarlet

    Plasmid

    #211330

    Purpose

    Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGTRm

    Plasmid

    #127197

    Purpose

    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA PCR template
    

    Use

    Template plasmid for generation of golden gate pc…

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMRX-IPU-mTagBFP2-Sec61B

    Plasmid

    #228136

    Purpose

    Expresses mTagBFP2-Sec61B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Sec61B (SEC61B Human)
    

    Use

    Retroviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXs-IP-PEX3-EGFP

    Plasmid

    #228142

    Purpose

    Expresses PEX3-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. I48 ELP

    Plasmid

    #68937

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

    Depositor

    Andrew MacKay

    Article

    Shi et al J Control Release. 2013 Nov 10;171(3):33

    Insert

    I48 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRX-IBU-SNAP-Rab7

    Plasmid

    #228141

    Purpose

    Expresses SNAP-Rab7 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Rab7 (Rab7 Mouse)
    

    Use

    Retroviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFsynW SYT1 reporter

    Plasmid

    #197275

    Purpose

    Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-HiBiT-CD63

    Plasmid

    #162591

    Purpose

    N-terminally HiBiT-tagged human CD63 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD63 (CD63 Synthetic, human, Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBa.GFP-KIF13B

    Plasmid

    #134626

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pF(UG) hSyn SYT7alpha-HaloTag

    Plasmid

    #179715

    Purpose

    Lentiviral expression of mouse Syt7 with C terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAVS-HsProm1s1-StayGold

    Plasmid

    #211333

    Purpose

    Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    AAV

    Tags

    StayGold

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. σ1-mCherry

    Plasmid

    #186578

    Purpose

    Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP1S1 (AP1S1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. gWiz_BM40-vGAT-VL-hIgG1-CL

    Plasmid

    #218437

    Purpose

    mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

    Depositor

    Axel Brunger

    Article

    Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

    Insert

    vGAT-VL-hIgG1-CL
    

    Use

    Affinity Reagent/ Antibody

    Tags

    BM40

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

    Plasmid

    #86424

    Purpose

    PCR tagging module with yeast codon optimized mTurquoise2

    Depositor

    Sue Jaspersen

    Article

    Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAD-CMV-STAT5A-CA-CMV-GFP

    Plasmid

    #83255

    Purpose

    Adenoviral expression of constitutively active STAT5A with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5a (Stat5a Mouse)
    eGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    constitutively active

    Promoter

    CMV

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge