Skip to main content
Addgene
Showing: 1001 - 1050 of 17298 results

  2. PF4-bio-His

    Plasmid
    #53411
    Purpose
    Expresses full-length Platelet factor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PF4 (PF4 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53411-plasmid-map-sequence-id-83194

    Enlarge

  3. THBS1-bio-His

    Plasmid
    #53417
    Purpose
    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    THBS1 (THBS1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53417-plasmid-map-sequence-id-83207

    Enlarge

  4. pENTR_L1-YFP-miniTurbo-L2

    Plasmid
    #127352
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127352-plasmid-map-sequence-id-246314

    Enlarge

  10. MTDH-His-bio

    Plasmid
    #52331
    Purpose
    Expresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    MTDH (MTDH Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52331-plasmid-map-sequence-id-81050

    Enlarge

  15. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid
    #127349
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127349-plasmid-map-sequence-id-246309

    Enlarge

  21. pGH00.0126

    Plasmid
    #64257
    Purpose
    Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation
    Depositor
    Mark Guiltinan, Siela Maximova
    Article
    Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E
    Inserts
    EGFP
    neomycin phosphotransferase II
    Use
    Binary agrobacterium vector for plant transformat…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    E12-omega
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64257-plasmid-map-sequence-id-115529

    Enlarge

  22. TFRC-His-bio

    Plasmid
    #52338
    Purpose
    Expresses full-length Transferrin receptor protein 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TFRC (TFRC Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52338-plasmid-map-sequence-id-81059

    Enlarge

  23. VTN-bio-His

    Plasmid
    #53429
    Purpose
    Expresses full-length Vitronectin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    VTN (VTN Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53429-plasmid-map-sequence-id-83219

    Enlarge

  24. pLV-REPORT(PGK/CMV)-GtwyA

    Plasmid
    #208574
    Purpose
    LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    mnucTomato
    HSVtk
    Neomycin
    d2nucEGFP
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/CMV and minP
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208574-plasmid-map-sequence-id-413226

    Enlarge

  25. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

    Plasmid
    #127362
    Purpose
    gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127362-plasmid-map-sequence-id-246307

    Enlarge

  27. pENTR_L1-Turbo-YFP-NLS-STOP-L2

    Plasmid
    #127361
    Purpose
    gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127361-plasmid-map-sequence-id-246302

    Enlarge

  31. pMuLE Lenti Dest Neo

    Plasmid
    #62178
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Lentiviral; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62178-plasmid-map-sequence-id-101462

    Enlarge

  32. SELP-bio-His

    Plasmid
    #51639
    Purpose
    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SELP (SELP Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51639-plasmid-map-sequence-id-78961

    Enlarge

  41. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid
    #127358
    Purpose
    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127358-plasmid-map-sequence-id-246304

    Enlarge

  42. SORT1-bio-His

    Plasmid
    #52024
    Purpose
    Expresses full-length Sortilin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SORT1 (SORT1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52024-plasmid-map-sequence-id-80096

    Enlarge

  45. pCSF107mT-GATEWAY-3'-LAP tag

    Plasmid
    #67618
    Purpose
    A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology
    Depositor
    Todd Stukenberg
    Article
    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi
    Type
    Empty backbone
    Use
    In vitro transcription or translation off of an s…
    Tags
    C-termnal LAP (eGFP-TEV-S)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and SP6
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  46. RH5-bio

    Plasmid
    #47780
    Purpose
    Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH5
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47780-plasmid-map-sequence-id-68799

    Enlarge

  50. TIMP1-bio-His

    Plasmid
    #52089
    Purpose
    Expresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TIMP1 (TIMP1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52089-plasmid-map-sequence-id-80365

    Enlarge

Showing: 1001 - 1050 of 17298 results